CAJ | 학술논문

目的：通过观察 A-NK细胞对裸鼠舌鳞状细胞癌移植瘤模型的体外生长特点以及口腔鳞状细胞癌（OS-CC）凋亡指数的检测，探讨 A-NK细胞对 OSCC的免疫治疗机制。方法建立裸鼠舌鳞癌皮下移植瘤模型，随机分为对照组、A-NK细胞组和 NA-NK细胞组，观察33天后瘤重，绘制肿瘤生长曲线；OSCC组织标本经末端脱氧核苷酸转移酶介导的 UTP缺口标记（TUNEL）染色以鉴定凋亡细胞，并由阳性细胞百分比表示细胞凋亡指数（AI）。结果 A-NK细胞组第15天开始肿瘤体积显著小于对照组（P＜0．05）；NA-NK细胞组第27天后肿瘤体积显著小于对照组（P＜0．05）；A-NK细胞组第30天肿瘤体积显著小于 NA-NK细胞组（P＜0．05），且三组荷瘤裸鼠肿瘤生长重量均有明显差异，差异有统计学意义（P＜0．05）。NK组病例活检癌组织 AI为（0．71±0．66）％，A-NK组织 AI为（1．76±0．83）％，二者间有显著性差异（P＜0．01）；对照组癌组织 AI为（0．34±0．69）％，与前二者间有显著性差异（P＜0．01）。结论 A-NK细胞和 NA-NK细胞均可在裸鼠体内诱导明显的抗肿瘤效应，且 A-NK的抑瘤作用大于 NA-NK细胞。NK细胞对口腔鳞状细胞癌具有免疫杀伤效应，该作用是通过诱导靶细胞凋亡实现的。
목적：통과관찰 A-NK세포대라서설린상세포암이식류모형적체외생장특점이급구강린상세포암（OS-CC）조망지수적검측，탐토 A-NK세포대 OSCC적면역치료궤제。방법건립라서설린암피하이식류모형，수궤분위대조조、A-NK세포조화 NA-NK세포조，관찰33천후류중，회제종류생장곡선；OSCC조직표본경말단탈양핵감산전이매개도적 UTP결구표기（TUNEL）염색이감정조망세포，병유양성세포백분비표시세포조망지수（AI）。결과 A-NK세포조제15천개시종류체적현저소우대조조（P＜0．05）；NA-NK세포조제27천후종류체적현저소우대조조（P＜0．05）；A-NK세포조제30천종류체적현저소우 NA-NK세포조（P＜0．05），차삼조하류라서종류생장중량균유명현차이，차이유통계학의의（P＜0．05）。NK조병례활검암조직 AI위（0．71±0．66）％，A-NK조직 AI위（1．76±0．83）％，이자간유현저성차이（P＜0．01）；대조조암조직 AI위（0．34±0．69）％，여전이자간유현저성차이（P＜0．01）。결론 A-NK세포화 NA-NK세포균가재라서체내유도명현적항종류효응，차 A-NK적억류작용대우 NA-NK세포。NK세포대구강린상세포암구유면역살상효응，해작용시통과유도파세포조망실현적。
Objective By observing the external growth and hyperplasia of A-NK cell ,To explore the treatment effect and mechanism of A-NK on the subcutaneous transplanted tumor of tongue squamous cell carcinoma in nude mice.And provide the experimental foundation in clinical use of such treatment strategies for tongue squamous carcino-ma.Methods The subcutaneous nude mice model with Tca8113 cells was established and randomly assigned to three groups .Every group had 7 nude mice.The control Group inj ected with normal saline,The experimental group inj ected with A-NK cells and NA-NKcells,All animals were killed after 33 days,The tumor volumes and weight in each group were measured and to draw the curve of tumor growth.Results The Volume of nude mice tumor in A-NK group is smaller than the control group after 1 5 days (P<0.05);The Volume of nude mice tumor in NA-NK group is smaller than the control Group after 27days(P<0.05);and the Volume of nude mice tumor in A-NK group is smaller than the NA-NK Group after 30 days(P<0.05),There were significant differences among the three groups in terms of the weight of nude mice tumor(P<0.05).Conclusion A-NK cells and NA-NK cells can significantly inhibit the subcuta-neous transplanted tumors of nude mice and the A-NK group is stronger than the NA-NK group.