CAJ | 학술논문

目的：探讨新霉素对脑胶质瘤细胞增殖及 PDGF、VEGF和血管生成素表达的影响。方法采用人脑胶质瘤细胞 U251，DMEM培养基添加10％胎牛血清培养。MTT细胞活性实验检测细胞增殖，Real Time PCR检测 mR-NA表达情况，酶联免疫吸附实验检测蛋白表达。结果 MTT结果表明，新霉素对 U251细胞增殖存在抑制作用，并以剂量依赖的方式进行，并发现新霉素的抑制作用随时间增强。Real Time PCR结果显示，新霉素作用后 U251细胞中PDGF、VEGF和血管生成素 mRNA 的表达都受到不同程度的抑制，分别降低了26．75％、38．23％和43．87％。ELISA分析表明新霉素能够降低PDGF、VEGF和血管生成素蛋白水平的表达。结论新霉素能够抑制脑胶质瘤细胞 U251中PDGF、VEGF和血管生成素的表达并抑制脑胶质瘤细胞增殖。
목적：탐토신매소대뇌효질류세포증식급 PDGF、VEGF화혈관생성소표체적영향。방법채용인뇌효질류세포 U251，DMEM배양기첨가10％태우혈청배양。MTT세포활성실험검측세포증식，Real Time PCR검측 mR-NA표체정황，매련면역흡부실험검측단백표체。결과 MTT결과표명，신매소대 U251세포증식존재억제작용，병이제량의뢰적방식진행，병발현신매소적억제작용수시간증강。Real Time PCR결과현시，신매소작용후 U251세포중PDGF、VEGF화혈관생성소 mRNA 적표체도수도불동정도적억제，분별강저료26．75％、38．23％화43．87％。ELISA분석표명신매소능구강저PDGF、VEGF화혈관생성소단백수평적표체。결론신매소능구억제뇌효질류세포 U251중PDGF、VEGF화혈관생성소적표체병억제뇌효질류세포증식。
Objective To observe the effect of neomycin on cell proliferation and expression of PDGF,VEGF,angio-genin in U251 glioma cells.Methods The cell proliferation was analyzed using MTT.Real Time PCR and ELISA were applied to investigate the expression of PDGF,VEGF,angiogenin.Results The MTT results showed that neomycin positively inhibited the cell proliferation of U251 cells and the inhibition was enhanced by dose-dependent and time-de-pendent.Real Time PCR and ELISA results showed that neomycin inhibited the expression of PDGF,VEGF,angiogenin both on mRNA level and protein level.Conclusion Neomycin positively inhibited the cell proliferation and expression of PDGF,VEGF,angiogenin in U251 glioma cells.