CAJ | 학술논문

建立了一种利用高效液相色谱法检测葡萄糖、麦芽糖和海藻糖混合溶液中海藻糖的方法。采用X bridge-N H 2色谱柱，流动相为乙腈/水=4∶1加0.1%的氨水，流速1.0 m L/m in，柱温35℃，该条件下葡萄糖、麦芽糖、海藻糖能完全分开，葡萄糖、麦芽糖、海藻糖的质量浓度在0.1～4.0 g/100 m L范围与峰面积呈良好线性关系，相关系数R 2为0.9998～0.9999，相对标准偏差为1.60%，回收率为98.2%～99.4%。
건립료일충이용고효액상색보법검측포도당、맥아당화해조당혼합용액중해조당적방법。채용X bridge-N H 2색보주，류동상위을정/수=4∶1가0.1%적안수，류속1.0 m L/m in，주온35℃，해조건하포도당、맥아당、해조당능완전분개，포도당、맥아당、해조당적질량농도재0.1～4.0 g/100 m L범위여봉면적정량호선성관계，상관계수R 2위0.9998～0.9999，상대표준편차위1.60%，회수솔위98.2%～99.4%。
AHPLC m ethod forthe detection oftrehalose in the m ix solution ofglucose,m altose and trehalose w as established.The sam ple w as sepa-rated by X bridge-N H 2 chrom atographic colum n,using acetonitrile:w ater= 4∶1 supplem ented w ith 0. 1% NH4OH as the m obile phase,the flow rate 1.0 ml/m in,the colum n tem perature 35 ℃.U nderthis condition,glucose,m altose,and trehalose w ere com pletely separated.W hen the concentration ofglucose,m altose,trehalose w asin the range of0.1-4.0 g/100 ml,the concentration and the peak area had a good linearrelationship,R2 was0.999 8-0.999 9,relative standard deviation w as1.60% ,and the recovery rate w as98.2% -99.4% .