中国酿造
中國釀造
중국양조
CHINA BREWING
2014年
10期
133-135
,共3页
西瓜%合成色素%甜味剂%超高效液相色谱
西瓜%閤成色素%甜味劑%超高效液相色譜
서과%합성색소%첨미제%초고효액상색보
w aterm elon%synthetic colors%sw eeteners%U PLC
建立了同时检测西瓜中4种合成色素和2种甜味剂的超高效液相色谱（U PLC）法。采用W atersA tlantisT 3色谱柱，以乙酸铵溶液（0.02 m ol/L）和甲醇为流动相，梯度洗脱，变波长扫描，各化合物分离效果好，线性关系良好（R 2＞0.999），6种添加剂的回收率在91.8%～99.8%，相对标准偏差在0.53%～3.87%，检出限在0.00015～0.00060μg/m L。表明该方法精密度高，准确度好，可以满足检测的需要。
建立瞭同時檢測西瓜中4種閤成色素和2種甜味劑的超高效液相色譜（U PLC）法。採用W atersA tlantisT 3色譜柱，以乙痠銨溶液（0.02 m ol/L）和甲醇為流動相，梯度洗脫，變波長掃描，各化閤物分離效果好，線性關繫良好（R 2＞0.999），6種添加劑的迴收率在91.8%～99.8%，相對標準偏差在0.53%～3.87%，檢齣限在0.00015～0.00060μg/m L。錶明該方法精密度高，準確度好，可以滿足檢測的需要。
건립료동시검측서과중4충합성색소화2충첨미제적초고효액상색보（U PLC）법。채용W atersA tlantisT 3색보주，이을산안용액（0.02 m ol/L）화갑순위류동상，제도세탈，변파장소묘，각화합물분리효과호，선성관계량호（R 2＞0.999），6충첨가제적회수솔재91.8%～99.8%，상대표준편차재0.53%～3.87%，검출한재0.00015～0.00060μg/m L。표명해방법정밀도고，준학도호，가이만족검측적수요。
A new m ethod for determ ination of 4 synthetic colors and 2sw eeteners in w aterm elon w as developed by ultra perform ance liquid chro-m atography (UPLC ).In this m ethod,W aters A tlantis T3 column was used to ensure good resolution,using am m onium acetate solution (0.02 mol/L) and m ethanolasm obile phase to conductgradientelution,and the detection w avelength w asvariable w ith the change ofcom pounds.The calibration curves show ed good linearity w ith correlation coefficients greaterthan 0.999.The optim ized m ethod allowed forrecoveries of91.8% -99.8% forsix additivesw ith relative standard deviation valuesof0.53% -3.87% ,and the lim itofdetection w as low in the range of0.000 15~0.000 60 μg/m l.The m ethod w asaccurate,in high precision w hich can m eetthe detecting requirem ent.
