CAJ | 학술논문

该文探讨藤茶活性成分二氢杨梅素（DMY）对明胶酶酶活性和酶蛋白表达量的影响。通过收集不同质量浓度DMY处理的人乳腺癌细胞的条件化培养液上清液，取等蛋白质含量各处理组样本液，进行相同条件的明胶酶谱实验，以此检测DMY对明胶酶蛋白表达量的影响；收集未经过DMY处理的细胞培养液上清液，等量体积上样进行明胶酶谱实验的SDS-PAGE电泳部分，移除凝胶中SDS后置于含有不同质量浓度DMY的孵育液中进行孵育，以此检测DMY对明胶酶活性的影响。结果表明，20～80μg/mL的DMY对明胶酶活性未见明显影响，但以剂量依赖方式抑制明胶酶蛋白的表达。这表明DMY抑制肿瘤转移的作用机制可能是通过抑制明胶酶酶蛋白的表达量，而不是通过抑制该酶活性来完成的。
해문탐토등다활성성분이경양매소（DMY）대명효매매활성화매단백표체량적영향。통과수집불동질량농도DMY처리적인유선암세포적조건화배양액상청액，취등단백질함량각처리조양본액，진행상동조건적명효매보실험，이차검측DMY대명효매단백표체량적영향；수집미경과DMY처리적세포배양액상청액，등량체적상양진행명효매보실험적SDS-PAGE전영부분，이제응효중SDS후치우함유불동질량농도DMY적부육액중진행부육，이차검측DMY대명효매활성적영향。결과표명，20～80μg/mL적DMY대명효매활성미견명현영향，단이제량의뢰방식억제명효매단백적표체。저표명DMY억제종류전이적작용궤제가능시통과억제명효매매단백적표체량，이불시통과억제해매활성래완성적。
The effectofdihydrom yricetin (DMY )on gelatinase activities and gelatinase proteins expression w as investigated.H um an breastcancer cellsw ere treated by differentconcentrationsofDMY .The conditioned m edia w ere collected,and equalam ountofproteinssam ple w ere analyzed by gelatin zym ography, w hich helped to detect the effect of DMY on gelatinase proteins expression. Supernatant w ithout DMY pretreatm ent w ere collected,and equalvolum e sam plesw ere loaded to conductSD S-PA G E partofthe gelatin enzym e spectrum experim ent.A fterrem ovalofSD S,the gels w ere incubated in incubation buffercontaining differentconcentrations ofD MY ,by w hich to detectthe effect of DMY on gelatinase activities. R esult show ed that 20-80 μg/m l DMY show ed no obvious effect on gelatinases activities, but inhibited the gelatinases proteins expression in a dose-dependentm anner.The DMY ’s anti-tum orm echanism could be by restraining the gelatinases proteins expression,ratherthan by inhibiting the gelatinase activities.