CAJ | 학술논문

利用葡萄枯枝蔓和甘蔗糖蜜酒精发酵液为主要培养基原料，发酵生产绿色木霉（Trichoderm a viride）孢子菌剂。以产孢量为测定指标，通过单因素试验优化了培养基的组成，并测试了发酵基质不灭菌对于木霉发酵的影响。研究结果表明：葡萄枯枝蔓和糖蜜酒精发酵液适宜作为生产绿色木霉的廉价培养基，优化后培养条件为固态基质粒径10～40目，糖蜜酒精发酵液中硫酸铵添加量1.0%，糖蜜酒精发酵液浓度5°B x，培养基固液混合比2.5∶1（g∶m L）。在此条件下，绿色木霉JK -20直接发酵未灭菌培养基产孢子量可达1.12×1010 C FU /g。
이용포도고지만화감자당밀주정발효액위주요배양기원료，발효생산록색목매（Trichoderm a viride）포자균제。이산포량위측정지표，통과단인소시험우화료배양기적조성，병측시료발효기질불멸균대우목매발효적영향。연구결과표명：포도고지만화당밀주정발효액괄의작위생산록색목매적렴개배양기，우화후배양조건위고태기질립경10～40목，당밀주정발효액중류산안첨가량1.0%，당밀주정발효액농도5°B x，배양기고액혼합비2.5∶1（g∶m L）。재차조건하，록색목매JK -20직접발효미멸균배양기산포자량가체1.12×1010 C FU /g。
Trichoderm a viride spore culture w as produced using grape branches and m olasses alcoholferm entation m ash as m ain m edium m aterial. U sing spore production as index,the m edium com position w as optim ized by single factor test,and the effectofnon-sterilization ferm entation sub-strate on T.viride ferm entation w as investigated.The resultshow ed thatthe low -costm edium ofgrape branches and m olasses alcoholferm entation m ash w assuitable forT.viride ferm entation.The optim alm edium w asobtained asfollow s:particle size ofthe solid substrate 10-40 m esh,(N H 4)2SO 4 1.0% in m olasses alcoholferm entation m ash,m olasses alcoholferm entation m ash 5 °B x and solid-liquid ratio 2.5∶1(g∶m l).U nderthiscondition,the spore production ofT.viride JK -20 by ferm enting non-sterilized m edium w as1.12×1010 C FU /g.