中国酿造
中國釀造
중국양조
CHINA BREWING
2014年
10期
32-35
,共4页
丁苏%张劲%张瑛%杨莹%管敬喜%仁德%李玮
丁囌%張勁%張瑛%楊瑩%管敬喜%仁德%李瑋
정소%장경%장영%양형%관경희%인덕%리위
葡萄枝蔓%糖蜜酒精发酵液%绿色木霉%固态发酵
葡萄枝蔓%糖蜜酒精髮酵液%綠色木黴%固態髮酵
포도지만%당밀주정발효액%록색목매%고태발효
grape branches%m olassesalcoholferm entation m ash%Trichoderm a viride%solid-substrate ferm entation
利用葡萄枯枝蔓和甘蔗糖蜜酒精发酵液为主要培养基原料,发酵生产绿色木霉(Trichoderm a viride)孢子菌剂。以产孢量为测定指标,通过单因素试验优化了培养基的组成,并测试了发酵基质不灭菌对于木霉发酵的影响。研究结果表明:葡萄枯枝蔓和糖蜜酒精发酵液适宜作为生产绿色木霉的廉价培养基,优化后培养条件为固态基质粒径10~40目,糖蜜酒精发酵液中硫酸铵添加量1.0%,糖蜜酒精发酵液浓度5°B x,培养基固液混合比2.5∶1(g∶m L)。在此条件下,绿色木霉JK -20直接发酵未灭菌培养基产孢子量可达1.12×1010 C FU /g。
利用葡萄枯枝蔓和甘蔗糖蜜酒精髮酵液為主要培養基原料,髮酵生產綠色木黴(Trichoderm a viride)孢子菌劑。以產孢量為測定指標,通過單因素試驗優化瞭培養基的組成,併測試瞭髮酵基質不滅菌對于木黴髮酵的影響。研究結果錶明:葡萄枯枝蔓和糖蜜酒精髮酵液適宜作為生產綠色木黴的廉價培養基,優化後培養條件為固態基質粒徑10~40目,糖蜜酒精髮酵液中硫痠銨添加量1.0%,糖蜜酒精髮酵液濃度5°B x,培養基固液混閤比2.5∶1(g∶m L)。在此條件下,綠色木黴JK -20直接髮酵未滅菌培養基產孢子量可達1.12×1010 C FU /g。
이용포도고지만화감자당밀주정발효액위주요배양기원료,발효생산록색목매(Trichoderm a viride)포자균제。이산포량위측정지표,통과단인소시험우화료배양기적조성,병측시료발효기질불멸균대우목매발효적영향。연구결과표명:포도고지만화당밀주정발효액괄의작위생산록색목매적렴개배양기,우화후배양조건위고태기질립경10~40목,당밀주정발효액중류산안첨가량1.0%,당밀주정발효액농도5°B x,배양기고액혼합비2.5∶1(g∶m L)。재차조건하,록색목매JK -20직접발효미멸균배양기산포자량가체1.12×1010 C FU /g。
Trichoderm a viride spore culture w as produced using grape branches and m olasses alcoholferm entation m ash as m ain m edium m aterial. U sing spore production as index,the m edium com position w as optim ized by single factor test,and the effectofnon-sterilization ferm entation sub-strate on T.viride ferm entation w as investigated.The resultshow ed thatthe low -costm edium ofgrape branches and m olasses alcoholferm entation m ash w assuitable forT.viride ferm entation.The optim alm edium w asobtained asfollow s:particle size ofthe solid substrate 10-40 m esh,(N H 4)2SO 4 1.0% in m olasses alcoholferm entation m ash,m olasses alcoholferm entation m ash 5 °B x and solid-liquid ratio 2.5∶1(g∶m l).U nderthiscondition,the spore production ofT.viride JK -20 by ferm enting non-sterilized m edium w as1.12×1010 C FU /g.