中国酿造
中國釀造
중국양조
CHINA BREWING
2014年
10期
14-17
,共4页
地衣芽孢杆菌%分离鉴定%生理生化特性%16S rDNA
地衣芽孢桿菌%分離鑒定%生理生化特性%16S rDNA
지의아포간균%분리감정%생리생화특성%16S rDNA
B acilluslicheniform is%identification%physiologicaland biochem icalcharacteristics%16S rD N A
为了探讨产乳酸的芽孢杆菌对青贮发酵的作用。以青贮三个月的豆科牧草疏叶骆驼刺为材料，进行MRS培养基分离、纯化细菌，革兰氏染色，芽孢染色，细胞形态学观察，生理生化特性鉴定和16SrDNA序列分析。结果显示，菌株杆状，G+，有芽孢，产乳酸；16SrDNA测序结果显示该菌株与模式菌株ATCC14580的相似性为99.3%。结果表明，该菌为地衣芽孢杆菌（Bacilluslicheniformis）。
為瞭探討產乳痠的芽孢桿菌對青貯髮酵的作用。以青貯三箇月的豆科牧草疏葉駱駝刺為材料，進行MRS培養基分離、純化細菌，革蘭氏染色，芽孢染色，細胞形態學觀察，生理生化特性鑒定和16SrDNA序列分析。結果顯示，菌株桿狀，G+，有芽孢，產乳痠；16SrDNA測序結果顯示該菌株與模式菌株ATCC14580的相似性為99.3%。結果錶明，該菌為地衣芽孢桿菌（Bacilluslicheniformis）。
위료탐토산유산적아포간균대청저발효적작용。이청저삼개월적두과목초소협락타자위재료，진행MRS배양기분리、순화세균，혁란씨염색，아포염색，세포형태학관찰，생리생화특성감정화16SrDNA서렬분석。결과현시，균주간상，G+，유아포，산유산；16SrDNA측서결과현시해균주여모식균주ATCC14580적상사성위99.3%。결과표명，해균위지의아포간균（Bacilluslicheniformis）。
In order to explore the effect of lactic acid-producing Bacillus on silage ferm entation, using Alhagi sparsifolia Shap. ensiled for three months as materials, Bacillus was isolated and purified with MRS medium. And then the isolate strains were identified with Gram staining,spore staining,cellcolony m orphology observation,physiologicaland biochem icalcharacteristics identification and 16S rDNA sequencing analysis wereused to identify. Result showed that the bacterium were rod-shaped, Gram-positive,spore-forming, and lactic acid-producing.The 16S rDNA result show ed thatthe similarity between the strain and the published sequence (type strain ATCC14580)was99.3%. To sum up,the strain wasidentified as Bacilluslicheniform is.
