中国医师杂志
中國醫師雜誌
중국의사잡지
JOURNAL OF CHINESE PHYSICIAN
2012年
z2期
4-6
,共3页
抗结核药/投药和剂量%疫苗,亚单位/投药和剂量%T淋巴细胞/病理生理学%小鼠
抗結覈藥/投藥和劑量%疫苗,亞單位/投藥和劑量%T淋巴細胞/病理生理學%小鼠
항결핵약/투약화제량%역묘,아단위/투약화제량%T림파세포/병리생이학%소서
Antitubercular agents/administration & dosage%Vaccines,subunit/administration &dosage%T-lymphocytes/physiopathology%Mice
目的 探索不同加强免疫策略对长期免疫记忆的影响,为结核疫苗的研究奠定理论基础.方法 将融合蛋白AMM、佐剂DDA和BCG-PSN混合构建AMM亚单位疫苗.选择50只C57BL/6小鼠(SPF级),按随机数字表法分为5组,每组10只,A组给小鼠注射磷酸盐缓冲液(PBS)作为对照;B组小鼠仅注射卡介苗(BCG)进行初免;C组小鼠BCG初免后,于第10周用AMM亚单位疫苗加强免疫;D组小鼠BCG初免后,分别于第8周、第10周用AMM亚单位疫苗加强免疫小鼠一次;E组小鼠BCG初免后,分别于第6周、第8周、第10周用AMM亚单位疫苗加强免疫小鼠一次.于第28周取出小鼠脾脏淋巴细胞,加入2.5μg/ml的Ag85B刺激72h后收集脾细胞培养上清,用ELISA测定Ag85B特异性脾淋巴细胞产生IFN-γ水平.结果 脾细胞受抗原Ag85B刺激后,BCG初免小鼠B组血清IFN-γ[(0.008±0.010)pg/ml]水平升高,但低于C组[血清IFN-γ(1.834±1.058)pg/ml]、D组[血清IFN-γ(2.337±0.964)pg/ml]、E组[血清IFN-γ(2.481±0.757) pg/ml],其差异均有统计学意义(P ＜0.05或P＜0.01),而A组血清IFN-γ[(0.005±0.048) pg/ml]水平均低于各免疫组(P ＜0.05或P＜0.01);C组血清IFN-γ水平均低于D、E两组,其差异有统计学意义(P＜0.05或P＜0.01);D组血清IFN-γ水平与E组比较差异无统计学意义(P＞0.05).结论 小鼠加强免疫强化的次数并非越多越好,适当的加强免疫次数可以诱导最持久的免疫记忆,亚单位疫苗加强免疫策略可能是影响免疫记忆的一个重要因素.
目的 探索不同加彊免疫策略對長期免疫記憶的影響,為結覈疫苗的研究奠定理論基礎.方法 將融閤蛋白AMM、佐劑DDA和BCG-PSN混閤構建AMM亞單位疫苗.選擇50隻C57BL/6小鼠(SPF級),按隨機數字錶法分為5組,每組10隻,A組給小鼠註射燐痠鹽緩遲液(PBS)作為對照;B組小鼠僅註射卡介苗(BCG)進行初免;C組小鼠BCG初免後,于第10週用AMM亞單位疫苗加彊免疫;D組小鼠BCG初免後,分彆于第8週、第10週用AMM亞單位疫苗加彊免疫小鼠一次;E組小鼠BCG初免後,分彆于第6週、第8週、第10週用AMM亞單位疫苗加彊免疫小鼠一次.于第28週取齣小鼠脾髒淋巴細胞,加入2.5μg/ml的Ag85B刺激72h後收集脾細胞培養上清,用ELISA測定Ag85B特異性脾淋巴細胞產生IFN-γ水平.結果 脾細胞受抗原Ag85B刺激後,BCG初免小鼠B組血清IFN-γ[(0.008±0.010)pg/ml]水平升高,但低于C組[血清IFN-γ(1.834±1.058)pg/ml]、D組[血清IFN-γ(2.337±0.964)pg/ml]、E組[血清IFN-γ(2.481±0.757) pg/ml],其差異均有統計學意義(P ＜0.05或P＜0.01),而A組血清IFN-γ[(0.005±0.048) pg/ml]水平均低于各免疫組(P ＜0.05或P＜0.01);C組血清IFN-γ水平均低于D、E兩組,其差異有統計學意義(P＜0.05或P＜0.01);D組血清IFN-γ水平與E組比較差異無統計學意義(P＞0.05).結論 小鼠加彊免疫彊化的次數併非越多越好,適噹的加彊免疫次數可以誘導最持久的免疫記憶,亞單位疫苗加彊免疫策略可能是影響免疫記憶的一箇重要因素.
목적 탐색불동가강면역책략대장기면역기억적영향,위결핵역묘적연구전정이론기출.방법 장융합단백AMM、좌제DDA화BCG-PSN혼합구건AMM아단위역묘.선택50지C57BL/6소서(SPF급),안수궤수자표법분위5조,매조10지,A조급소서주사린산염완충액(PBS)작위대조;B조소서부주사잡개묘(BCG)진행초면;C조소서BCG초면후,우제10주용AMM아단위역묘가강면역;D조소서BCG초면후,분별우제8주、제10주용AMM아단위역묘가강면역소서일차;E조소서BCG초면후,분별우제6주、제8주、제10주용AMM아단위역묘가강면역소서일차.우제28주취출소서비장림파세포,가입2.5μg/ml적Ag85B자격72h후수집비세포배양상청,용ELISA측정Ag85B특이성비림파세포산생IFN-γ수평.결과 비세포수항원Ag85B자격후,BCG초면소서B조혈청IFN-γ[(0.008±0.010)pg/ml]수평승고,단저우C조[혈청IFN-γ(1.834±1.058)pg/ml]、D조[혈청IFN-γ(2.337±0.964)pg/ml]、E조[혈청IFN-γ(2.481±0.757) pg/ml],기차이균유통계학의의(P ＜0.05혹P＜0.01),이A조혈청IFN-γ[(0.005±0.048) pg/ml]수평균저우각면역조(P ＜0.05혹P＜0.01);C조혈청IFN-γ수평균저우D、E량조,기차이유통계학의의(P＜0.05혹P＜0.01);D조혈청IFN-γ수평여E조비교차이무통계학의의(P＞0.05).결론 소서가강면역강화적차수병비월다월호,괄당적가강면역차수가이유도최지구적면역기억,아단위역묘가강면역책략가능시영향면역기억적일개중요인소.
Objective Exploring different strengthening immune strategy on long-term immune memory effects for tuberculosis vaccine lays the theoretical foundation.Methods The fusion protein AMM,adjuvant DDA and BCG-PSN mixed built AMM subunit vaccine.Choose only 50 C57BL/6 mice (SPF),according to the table of random number method was divided into 5 groups and each group 10 only,the experiment 1 group injected mice to phosphate buffer solution (PBS) as control; Experiment 2 mice only injected BCG vaccine (BCG) from early; Experiment 3 mice BCG initial free,in 10 weeks with AMM subunit vaccine strengthen immunity; The four mice from early after BCG,respectively on 8 weeks,10 weeks with AMM subunit vaccine strengthen immune mice one time.Experimental 5 groups of mice after BCG initial free,respectively on 6 weeks,8 weeks,10 weeks with AMM subunit vaccine strengthen immune mice a.28 weeks to remove spleen lymphocytes in mice,add 2.5μg/ml Ag85B stimulus 72 h after collecting spleen cell culture supernatant,with ELISA test Ag85B specificity splenic lymphocyte caused IFN ppar-gamma level.Results Spleen cells by antigen Ag85B stimulation,BCG initial free,AMM subunit vaccine strengthen immune once,twice,three times group and PBS compared all had difference (P ＜0.01 orP ＜0.05) ;BCG initial free-AMM subunit vaccine strengthen immune once,twice,three times group and BCG compared all had difference (P ＜0.01 or P ＜0.05) ; BCG initial free-AMM subunit vaccine strengthen immune once and twice or three times group compared with difference (P ＜0.01 orP ＜0.05) ; Initial free-AMM subunit vaccine strengthen immune two group and three times than group difference was statistically significant (P ＞0.05).Conclusion Mice strong immune strengthened the number is not the more the better,the appropriate number of strengthen the immune to induce the most lasting immunity memory,subunit vaccine strengthen immunization strategy may affect the immune memory is one of the important factors.
