泰山医学院学报
泰山醫學院學報
태산의학원학보
JOURNAL OF TAISHAN MEDICAL COLLEGE
2014年
10期
984-988
,共5页
富氢水%肝脏%缺血再灌注损伤%实验
富氫水%肝髒%缺血再灌註損傷%實驗
부경수%간장%결혈재관주손상%실험
hydrogen - rich suline%liver%schemia - reperfusion injury%experimental study
目的：探讨富氢水预处理对大鼠肝缺血再灌注损伤的影响。方法健康雄性 SD 大鼠30只，质量220~250 g，釆用随机数字表法分为3组（n =10），假手术组（Sham 组）、缺血再灌注组（ IR 组）和富氢水组（ HS 组）。Sham 组仅暴露肝门，不夹闭动、静脉；IR 组采用夹闭左、中叶肝肝动脉支60 min（保留门静脉血流），然后恢复灌注，制备大鼠肝缺血再灌注模型；HS 组于手术前3天口服2%富氢水（40 ml·kg -1·d -1），其余两组口服同等剂量生理盐水。各组于再灌注6 h 抽取下腔静脉血样并取肝组织，采用全自动生化分析仪测定血清谷丙转氨酶（ ALT）和谷草转氨酶（AST）活性，光镜下观察肝组织病理学变化。结果与 Sham 组相比，IR 组血清 ALT 和 AST 升高（P <0.05）；与 IR 组相比，HS 组 ALT 和 AST 活性降低（P <0.05）；HS 组肝病理学损伤较 IR 组明显减轻。结论硫化氢后处理可减轻大鼠肝缺血再灌注损伤。
目的：探討富氫水預處理對大鼠肝缺血再灌註損傷的影響。方法健康雄性 SD 大鼠30隻，質量220~250 g，釆用隨機數字錶法分為3組（n =10），假手術組（Sham 組）、缺血再灌註組（ IR 組）和富氫水組（ HS 組）。Sham 組僅暴露肝門，不夾閉動、靜脈；IR 組採用夾閉左、中葉肝肝動脈支60 min（保留門靜脈血流），然後恢複灌註，製備大鼠肝缺血再灌註模型；HS 組于手術前3天口服2%富氫水（40 ml·kg -1·d -1），其餘兩組口服同等劑量生理鹽水。各組于再灌註6 h 抽取下腔靜脈血樣併取肝組織，採用全自動生化分析儀測定血清穀丙轉氨酶（ ALT）和穀草轉氨酶（AST）活性，光鏡下觀察肝組織病理學變化。結果與 Sham 組相比，IR 組血清 ALT 和 AST 升高（P <0.05）；與 IR 組相比，HS 組 ALT 和 AST 活性降低（P <0.05）；HS 組肝病理學損傷較 IR 組明顯減輕。結論硫化氫後處理可減輕大鼠肝缺血再灌註損傷。
목적：탐토부경수예처리대대서간결혈재관주손상적영향。방법건강웅성 SD 대서30지，질량220~250 g，변용수궤수자표법분위3조（n =10），가수술조（Sham 조）、결혈재관주조（ IR 조）화부경수조（ HS 조）。Sham 조부폭로간문，불협폐동、정맥；IR 조채용협폐좌、중협간간동맥지60 min（보류문정맥혈류），연후회복관주，제비대서간결혈재관주모형；HS 조우수술전3천구복2%부경수（40 ml·kg -1·d -1），기여량조구복동등제량생리염수。각조우재관주6 h 추취하강정맥혈양병취간조직，채용전자동생화분석의측정혈청곡병전안매（ ALT）화곡초전안매（AST）활성，광경하관찰간조직병이학변화。결과여 Sham 조상비，IR 조혈청 ALT 화 AST 승고（P <0.05）；여 IR 조상비，HS 조 ALT 화 AST 활성강저（P <0.05）；HS 조간병이학손상교 IR 조명현감경。결론류화경후처리가감경대서간결혈재관주손상。
Objective:To investigate the effects of hydrogen - rich suline postcondi - tioning on hepatic ischemia -reperfusion injury in rats. Methods:Thirty healthy male SD rats weighing 220 ~ 250g were randomly divided into 3 groups(n= 10 each):Shame operaton group( Sham group),ischemia - reperfusion group( IR group)and hydrogen - rich suline groups(HS groups)In Sham group hepatic portal was only exposed but not ligated. IR group the left and middle lobes of he-patic pedicle,portal vein and hepatic artery were clamped for 60 min and then reperfused. HS groups 3 days before the sur-gery were received oral 2% rich hydrogen - rich water(40 ml·kg - 1 ·d - 1 ). Blood samples were collected for measuring the activity of alanine transaminase(ALT)and aspartate transaminase(AST)in serm at 6h after reperfusion. Liver tissue samples were collected for pathologic examination. Results :Compared with Sham group,the activities of ALT and AST was increased,Compared with IR group,the activities of ALT and AST in serm was decreased. Hepatic tissue pathologic injury was reduced in HS groups as compared with IR group. Conclusion:Hydrogen - rich suline preconditioning can reduce hc-patic ischemia - reperfusion injury in rats.
