实用医技杂志
實用醫技雜誌
실용의기잡지
JOURNAL OF PRACTICAL MEDICAL TECHNIQUES
2014年
10期
1045-1047
,共3页
超速离心法%视野%血型抗原
超速離心法%視野%血型抗原
초속리심법%시야%혈형항원
Ultracentrifugation%Visual fields%Blood group antigens
目的：分析毛细管超速离心技术对混合视野红细胞的分离能力。方法取来自上海血液中心献血员的新鲜A型血液5 mL、离体7 d和14 d的陈旧性B型血液各5 mL，分别用0.9%氯化钠注射液洗涤3次后制成压积红细胞，然后加入正常AB型人血浆使各标本细胞压积在80%左右，通过人为混合使陈旧性B细胞在新鲜A细胞中的比例为3%、6%，经过11000 r/min，5 min超速离心后分别取远、近心端细胞通过与一系列标准管进行比较来判读超速离心后远、近心端的细胞混合比例，并比较不同离体时间即陈旧程度不同的细胞对超速离心效果的影响，上述实验重复观察3次。结果2种比例混合样本（3%和6%）远心端B细胞所占百分比均明显高于初始混合细胞比例（Z=-2.121，P=0.034），新鲜细胞A细胞分布在近心端，陈旧B细胞被浓缩至远心端，可见超速离心法能够把新、旧细胞进行有效分离；离体7 d、14 d的陈旧细胞浓缩比例差异均不明显（Z=-1.826，P=0.068），超速离心后与离心前相比较B细胞百分比均增加了约1倍。结论毛细管超速离心技术对一些混合视野红细胞可以进行有效分离，值得在临床输血相关实验室中推广使用。
目的：分析毛細管超速離心技術對混閤視野紅細胞的分離能力。方法取來自上海血液中心獻血員的新鮮A型血液5 mL、離體7 d和14 d的陳舊性B型血液各5 mL，分彆用0.9%氯化鈉註射液洗滌3次後製成壓積紅細胞，然後加入正常AB型人血漿使各標本細胞壓積在80%左右，通過人為混閤使陳舊性B細胞在新鮮A細胞中的比例為3%、6%，經過11000 r/min，5 min超速離心後分彆取遠、近心耑細胞通過與一繫列標準管進行比較來判讀超速離心後遠、近心耑的細胞混閤比例，併比較不同離體時間即陳舊程度不同的細胞對超速離心效果的影響，上述實驗重複觀察3次。結果2種比例混閤樣本（3%和6%）遠心耑B細胞所佔百分比均明顯高于初始混閤細胞比例（Z=-2.121，P=0.034），新鮮細胞A細胞分佈在近心耑，陳舊B細胞被濃縮至遠心耑，可見超速離心法能夠把新、舊細胞進行有效分離；離體7 d、14 d的陳舊細胞濃縮比例差異均不明顯（Z=-1.826，P=0.068），超速離心後與離心前相比較B細胞百分比均增加瞭約1倍。結論毛細管超速離心技術對一些混閤視野紅細胞可以進行有效分離，值得在臨床輸血相關實驗室中推廣使用。
목적：분석모세관초속리심기술대혼합시야홍세포적분리능력。방법취래자상해혈액중심헌혈원적신선A형혈액5 mL、리체7 d화14 d적진구성B형혈액각5 mL，분별용0.9%록화납주사액세조3차후제성압적홍세포，연후가입정상AB형인혈장사각표본세포압적재80%좌우，통과인위혼합사진구성B세포재신선A세포중적비례위3%、6%，경과11000 r/min，5 min초속리심후분별취원、근심단세포통과여일계렬표준관진행비교래판독초속리심후원、근심단적세포혼합비례，병비교불동리체시간즉진구정도불동적세포대초속리심효과적영향，상술실험중복관찰3차。결과2충비례혼합양본（3%화6%）원심단B세포소점백분비균명현고우초시혼합세포비례（Z=-2.121，P=0.034），신선세포A세포분포재근심단，진구B세포피농축지원심단，가견초속리심법능구파신、구세포진행유효분리；리체7 d、14 d적진구세포농축비례차이균불명현（Z=-1.826，P=0.068），초속리심후여리심전상비교B세포백분비균증가료약1배。결론모세관초속리심기술대일사혼합시야홍세포가이진행유효분리，치득재림상수혈상관실험실중추엄사용。
Objective To analyze the capability of separating red blood cells with mixed field by ultracentrifu-gation technique. Methods Five mL fresh blood of type A was obtained from Shanghai Blood Center blood donors , meanwhile, 5 mL blood of type B was gotten, the preserving time of which was 7 d and 14 d in vitro respectively. All the three samples were washed three times with saline to make packed red blood cells , then added the normal plasma of type A and B, so that all samples were prepared to an appropriate hematocrit of 80%, next, the 3%, 6%of proportion of old cells in fresh A cell were reconstituted to simulate the mixed field artificially. The cell mixing ratio of proximal and distal end was read by comparing with a range of standard ratio after the samples were centrifuged with the speed of 11 000 r/min for 5 min, furthermore, the impact of different of preserving time in vitro on the effect of ultracentrifugation was also observed and all the above mentioned experiments were repeated three times. Results The percentage of B cells for the two samples (3%and 6%) was significantly higher than the initial mixing cell ratio (Z=-2.121, P=0.034) in the distal end, and fresh cells A cells were relocated in the proximal end, it demonstrated that the old and fresh red cells can be separated effectively by the ultracentrifugation technology. In addition , the difference of concentration ratio between 7 d and 14 d cells wasn′t significant(Z=-1.826, P=0.068), the previous percentage of B cells was increased about 1 times by ultracentrifugation. Conclusion The cells with mixed field can be separated effectively using the capillary ultracentr-ifugation technology and should be introduced in the clinical transfusion-related laboratories.
