CAJ | 학술논문

目的：观察金银花、山银花水提液不同配比(1∶0、2∶1、1∶1、1∶2、0∶1)对3种自由基的清除作用,以及对H2 O2诱导的内皮细胞氧化损伤的保护作用。方法采用1,1-二苯基-2-苦肼基自由基( DPPH·)、羟基自由基(·OH)、超氧阴离子自由基( O2-·[ O])清除法测定金银花-山银花水提液5个不同配比组的抗氧化活性；采用体外培养HUVECs细胞并复制细胞损伤模型,测定金银花-山银花水提液5个不同配比组受损细胞的活力、受损细胞上清中乳酸脱氢酶( LDH)和一氧化氮( NO)的含量。结果金银花-山银花水提液不同配比均有一定的抗氧化作用,其中金银花-山银花2∶1组最佳。金银花-山银花水提液不同配比能够使受损细胞NO的含量增加、LDH释放减少,其中金银花-山银花1∶0组作用最强,2∶1组次之。结论金银花-山银花不同配比在体外有较强的抗自由基和抗氧化作用,并且能够保护损伤的HUVECs细胞,金银花-山银花2∶1综合效果最佳。
목적：관찰금은화、산은화수제액불동배비(1∶0、2∶1、1∶1、1∶2、0∶1)대3충자유기적청제작용,이급대H2 O2유도적내피세포양화손상적보호작용。방법채용1,1-이분기-2-고정기자유기( DPPH·)、간기자유기(·OH)、초양음리자자유기( O2-·[ O])청제법측정금은화-산은화수제액5개불동배비조적항양화활성；채용체외배양HUVECs세포병복제세포손상모형,측정금은화-산은화수제액5개불동배비조수손세포적활력、수손세포상청중유산탈경매( LDH)화일양화담( NO)적함량。결과금은화-산은화수제액불동배비균유일정적항양화작용,기중금은화-산은화2∶1조최가。금은화-산은화수제액불동배비능구사수손세포NO적함량증가、LDH석방감소,기중금은화-산은화1∶0조작용최강,2∶1조차지。결론금은화-산은화불동배비재체외유교강적항자유기화항양화작용,병차능구보호손상적HUVECs세포,금은화-산은화2∶1종합효과최가。
Objective To observe the scavenging effect on 3 kinds of different freedom radicals and the pro-tective effect against the damage caused by hydrogen peroxide ( H2 O2 ) in endothelial cells of water extracts from hon-eysuckle flower and wild honeysuckle flower in different proportions (1∶0,2∶1,1∶1,1∶2,0∶1). Methods The scaven-ging ability of 5 different proportions of water extracts from honeysuckle flower and wild honeysuckle flower on 1,1-di-phenyl-2-trinitro bitter hydrazine radical (DPPH·),hydroxyl radical (·OH),superoxide anion radical (O2-·) was de-termined to evaluate the antioxidant activity of the water extracts from honeysuckle flower and wild honeysuckle flower in different proportions. HUVECs were cultured in vitro to build the hypoxic-reoxygenation model,then the cell viability was determined by MTT,and the lactate dehydrogenase ( LDH) and nitrogen monoxide ( NO) level in cell culture me-dium of 5 kinds of different proportions of water extracts from honeysuckle flower and wild honeysuckle flower were detected. Results All of the different proportions had the antioxidant effect,and the honeysuckle flower and wild hon-eysuckle flower 2∶1 group had the best effect. All different proportions could effectively release the contention of NO and reduce LDH release from damaged cells,and the honeysuckle flower and wild honeysuckle flower 1∶0 group had the best effect,followed by honeysuckle flower and wild honeysuckle flower 2∶1 group. Conclusion All different pro-portions of water extracts from honeysuckle flower and wild honeysuckle flower have obviously anti free radical and an-tioxidant effects in vitro,and have protective effect on human umbilical vein endothelial cells injury,and the honeysuck-le flower and wild honeysuckle flower 2∶1 group had the best effect.