CAJ | 학술논문

目的探讨氯沙坦对心肌缺血大鼠心肌组织中ACE2表达水平的影响。方法 SPF级健康雄性SD大鼠60只(体重220~240 g),通过部分结扎冠状动脉前降支建立大鼠心肌缺血模型,将术后24 h存活的42只大鼠随机分为14 d模型组、28 d模型组、14 d氯沙坦大剂量组、14 d氯沙坦小剂量组、28 d氯沙坦大剂量组、28 d氯沙坦小剂量组、假手术组,每组6只。术后24 h开始灌胃给药,小剂量氯沙坦组10 mg/( kg·d)灌胃给药,大剂量氯沙坦组30 mg/( kg·d)灌胃给药,假手术组及模型组给予等量生理盐水灌胃；分别于用药14 d、28 d后,行HE染色,明确心肌组织缺血病变情况,Western blot法检测左心室缺血区心肌组织ACE2蛋白表达的水平。结果模型组大鼠缺血心肌组织中ACE2蛋白表达量较假手术组升高( P<0．01),呈时间依赖性。氯沙坦剂量依赖性增加ACE2蛋白的表达量(P<0．05)。结论氯沙坦可增加心肌缺血后大鼠心肌组织中ACE2的表达量,进一步改善心肌缺血状态。
목적탐토록사탄대심기결혈대서심기조직중ACE2표체수평적영향。방법 SPF급건강웅성SD대서60지(체중220~240 g),통과부분결찰관상동맥전강지건립대서심기결혈모형,장술후24 h존활적42지대서수궤분위14 d모형조、28 d모형조、14 d록사탄대제량조、14 d록사탄소제량조、28 d록사탄대제량조、28 d록사탄소제량조、가수술조,매조6지。술후24 h개시관위급약,소제량록사탄조10 mg/( kg·d)관위급약,대제량록사탄조30 mg/( kg·d)관위급약,가수술조급모형조급여등량생리염수관위；분별우용약14 d、28 d후,행HE염색,명학심기조직결혈병변정황,Western blot법검측좌심실결혈구심기조직ACE2단백표체적수평。결과모형조대서결혈심기조직중ACE2단백표체량교가수술조승고( P<0．01),정시간의뢰성。록사탄제량의뢰성증가ACE2단백적표체량(P<0．05)。결론록사탄가증가심기결혈후대서심기조직중ACE2적표체량,진일보개선심기결혈상태。
Objective To investigate the effect of losartan on cardic ACE2 protein expression in myocardial ischemia rat. Methods Myocardial ischemia rat model was established by ligation of the anterior descending coronary artery. Twenty-four hours after the procedure,the 42 surviving rats were randomly divided into model with 14 or 28 d group (n=6),losartan large dose treatment with 14 or 28 d group (n=6),losartan low dose treatment with 14 or 28 d group (n=6) and Sham group (n=6). The rats in losartan large and low dose groups were treated respectively with 10 mg/( kg·d) and 30 mg/( kg·d) of losartan,rats in sham group were treated with equal quantity physiological sa-line. The pathological changes of myocardial was observed by HE staining,the relative expression of myocardium ACE2 protein was assessed by Western-blot. Results The protein expression of ACE2 in model group were increased com-pared with sham group in time dependence ( P<0. 05 ) . The ACE2 protein level of myocardiac with losartan treated group was significantly increased in dose-time dependence compared with the non-treated ones (P<0. 05). Conclusion Large-dose losartan can improve the myocardial ischemia status by increasing the ACE2 expression in myocardium of rats.