CAJ | 학술논문

目的：研究发酵培育的蝙蝠蛾拟青霉菌丝体（ PHM）抗疲劳、耐缺氧作用及其机制。方法将72只雄性昆明小鼠随机分为正常饲养组（ Home组）、对照组、莫达非尼（ Moda）组以及PHM低、中、高剂量组。给药14 d，每天2次。除Home组外，其他5组小鼠于末次给药后40 min，8、24和48 h通过轮式疲劳仪接受强迫攀跑训练，并于72 h接受力竭攀跑训练。记录小鼠每次训练过程中的电击次数。力竭训练结束后，运用试剂盒检测小鼠的肝糖原、肌糖原、血清尿素氮、血清乳酸、血清肌酸激酶和全血ATP水平。另将96只雄性昆明小鼠随机分为对照组和PHM低、中、高剂量组，每组24只，其中12只进行常压耐缺氧实验，另12只进行亚硝酸钠中毒实验。结果与对照组相比，PHM 能显著降低小鼠电击次数，改善疲劳相关生化指标并显著延长其缺氧存活时间。结论PHM作为虫草替代品用于抗疲劳、耐缺氧具有一定的潜力和良好的应用前景。
목적：연구발효배육적편복아의청매균사체（ PHM）항피로、내결양작용급기궤제。방법장72지웅성곤명소서수궤분위정상사양조（ Home조）、대조조、막체비니（ Moda）조이급PHM저、중、고제량조。급약14 d，매천2차。제Home조외，기타5조소서우말차급약후40 min，8、24화48 h통과륜식피로의접수강박반포훈련，병우72 h접수력갈반포훈련。기록소서매차훈련과정중적전격차수。력갈훈련결속후，운용시제합검측소서적간당원、기당원、혈청뇨소담、혈청유산、혈청기산격매화전혈ATP수평。령장96지웅성곤명소서수궤분위대조조화PHM저、중、고제량조，매조24지，기중12지진행상압내결양실험，령12지진행아초산납중독실험。결과여대조조상비，PHM 능현저강저소서전격차수，개선피로상관생화지표병현저연장기결양존활시간。결론PHM작위충초체대품용우항피로、내결양구유일정적잠력화량호적응용전경。
Objective To investigate the anti-fatigue and anti-hypoxia effect of fermented Paecilomyces hepiali myceli-um (PHM) and to explore its mechanism.Methods Seventy-two naive Kunming male mice were randomly divided into Home, vehicle, modafinil( Mode) , low-dose PHM, moderate-dose PHM, and high-dose PHM groups.All mice were ad-ministrated with the drug or vehicle twice a day during a 14-day period.Except for the Home group, each groups was forced into the wheel fatigue device to receive a climb-run training at the time points of 40 min, 8 h, 24 h and 48 h, and to re-ceive an exhaustive training at the time point of 72 h after the last administration.The number of electric shocks was recor-ded during each training and the levels of muscle glycogen, liver glycogen, serum urea nitrogen, serum lactic acid, serum creatine kinase and blood ATP were detected after the exhaustive training using commercially available kits.In addition, 96 naive Kunming male mice were randomly and equally divided into the vehicle, low-dose, moderate-dose and high-dose PHM groups.Twelve mice in each group were chosen for the normal pressure anti-hypoxia experiment and the other 12 mice were used for the NaNO3 poisoning experiment.Results Compared with the vehicle groups, the PHM group showed a sig-nificant decrease in the number of electric shocks, improvement in biochemical parameters associated with fatigue, and an increased survival time in the anti-hypoxia and the NaNO3 toxicity tests.Conclusion PHM is potentially an effective alter-native for wild Cordyceps in the treatment of fatigue and hypoxia.