CAJ | 학술논문

目的：探讨曲酸对辐照后小鼠免疫系统的影响。方法将20只雄性C57小鼠随机分为正常组、模型照射组、曲酸低、高剂量组。正常组动物不做任何处理，其他3组动物在照前27 h分别单次皮下注射无菌蒸馏水、75及300 mg/kg的曲酸溶液，注射体积为0．2 ml/20 g体质量。3组动物经4 Gy 60 Coγ射线单次照射。照后第2天及第8天分两批处死小鼠，分别测定小鼠的脾淋巴细胞转化能力及脾脏和胸腺指数，并制作组织病理学切片观察其形态学变化。结果与模型照射组比较，300 mg/kg曲酸给药组小鼠的脾淋巴细胞转化能力、脾脏和胸腺指数均显著提高（P＜0．01），而且脾脏和胸腺的组织形态学变化改善明显；与正常组比较，模型照射组小鼠的上述指标均显著受损。结论急性辐射可明显损伤小鼠的免疫系统；曲酸能够增强淋巴细胞的增殖能力，对辐照后小鼠的免疫系统有显著的保护作用。
목적：탐토곡산대복조후소서면역계통적영향。방법장20지웅성C57소서수궤분위정상조、모형조사조、곡산저、고제량조。정상조동물불주임하처리，기타3조동물재조전27 h분별단차피하주사무균증류수、75급300 mg/kg적곡산용액，주사체적위0．2 ml/20 g체질량。3조동물경4 Gy 60 Coγ사선단차조사。조후제2천급제8천분량비처사소서，분별측정소서적비림파세포전화능력급비장화흉선지수，병제작조직병이학절편관찰기형태학변화。결과여모형조사조비교，300 mg/kg곡산급약조소서적비림파세포전화능력、비장화흉선지수균현저제고（P＜0．01），이차비장화흉선적조직형태학변화개선명현；여정상조비교，모형조사조소서적상술지표균현저수손。결론급성복사가명현손상소서적면역계통；곡산능구증강림파세포적증식능력，대복조후소서적면역계통유현저적보호작용。
Objective To evaluate the effect of kojic acid( KA) on the immune system of mice after exposure to gam-ma-irradiation.Methods Twenty male C57BL/6 mice were divided into normal group, irradiation group, low/high doses of KA pretreated groups.Mice in normal group did not receive any treatment,while mice in other groups were sc injected with a single dose of sterile distilled water or KA(75 and 300 mg/kg, respectively) 27 h prior to a sublethal dose(4 Gy, 138.54 and 140.30 cGy/min, respectively) of whole body gamma-irradiation.The injected volume was calculated by 0.2 ml/20 g.Forty mice were sacrificed at day 2 and day 8 post-irradiation, respectively.The splenic lymphocyte transformation and spleen and thymus indexes were determined.Histopathological sections were produced, and the morphological changes were also observed.Results The splenic lymphocyte transformation capacity and spleen and thymus indexes of mice pre-treated with 300 mg/kg elatve mass KA were increased significantly ( P<0.01) compared with the irradiation group.The morphological changes in the spleen and thymus of mice in 300 mg/kg KA pretreated group were better than in the irradia-tion group.The above parameters of mice in irradiation group were injured severely in comparison with the normal group. Conclusion Acute radiation can damage the immune system of mice obviously.KA can enhance the transformation capaci-ty of lymphocytes and has marked protective effect on the immune system of mice after irradiation.