CAJ | 학술논문

以羧甲基纤维素酶活力、滤纸酶活力为考察指标，探讨绿色木霉（Trichoderma viride ）化L4C 和黑曲霉（Aspergillus niger ）混合固态发酵产纤维素酶的最佳培养时间；并以降解率为考察指标，通过单因素和正交试验对混菌固态发酵产纤维素酶条件进行优化，以促进秸秆资源的饲料化利用、缓解粮食危机，降低环境污染。结果表明，绿色木霉化 L4C 和黑曲霉混合固态发酵最佳时间为3 d，最佳总接种量为10％，绿色木霉化 L4C 与黑曲霉的最佳接种比例为1∶1，培养基最佳氮源为硫酸铵，麸皮与稻草秸秆粉最佳质量比为3∶7，最适宜培养基含水量为50％。在该条件下稻草秸秆半纤维素降解率可达34·83％，纤维素降解率可达39·75％，木质素降解率可达27·41％。
이최갑기섬유소매활력、려지매활력위고찰지표，탐토록색목매（Trichoderma viride ）화L4C 화흑곡매（Aspergillus niger ）혼합고태발효산섬유소매적최가배양시간；병이강해솔위고찰지표，통과단인소화정교시험대혼균고태발효산섬유소매조건진행우화，이촉진갈간자원적사료화이용、완해양식위궤，강저배경오염。결과표명，록색목매화 L4C 화흑곡매혼합고태발효최가시간위3 d，최가총접충량위10％，록색목매화 L4C 여흑곡매적최가접충비례위1∶1，배양기최가담원위류산안，부피여도초갈간분최가질량비위3∶7，최괄의배양기함수량위50％。재해조건하도초갈간반섬유소강해솔가체34·83％，섬유소강해솔가체39·75％，목질소강해솔가체27·41％。
The optimum fermentation time of solid fermentation of mixed Trichoderma viride L4C and Aspergillus niger with filter paper activity and carboxymethyl cellulase activity as indexes was studied,and the solid fermentation conditions were optimized by single factor test and orthogonal experiments with degradation rate as index,so as to promote the utilization of straw for fodder,relieving food crisis,reducing the environmental pollution.The optimum solid fermentation conditions were fermentation time of 3 d,total inoculum volume of 10%,inoculation ratio of Trichoderma viride L4C to Aspergillus niger of 1 ∶ 1,(NH4 )2 SO4 as nitrogen source, mass ratio of bran to straw stalk powder of 3 ∶ 7,moisture contents of 50%.Under the above conditions,the degradation rate of hemicellulose,cellulose,lignin for straw stalk were 34·83%, 39·75% and 27·41%.