CAJ | 학술논문

为了研究野桑蚕乳酸脱氢酶基因的功能，选择野桑蚕5龄3日幼虫的组织及不同发育时期的个体，利用 PCR 技术对野桑蚕乳酸脱氢酶基因进行克隆，得到野桑蚕乳酸脱氢酶基因开放阅读框长度为996 bp，没有内含子，只有1个外显子。该基因编码的蛋白含有331个氨基酸残基，等电点为6·76，分子量为36·35 kD。氨基酸序列分析表明，该蛋白高度保守，亚细胞定位预测分析表明该蛋白主要定位于细胞质中。转录表达分析表明，该基因在野桑蚕5龄3日幼虫的组织（马氏管、中肠、表皮、脂肪体、丝腺、血液、精巢和卵巢）及不同发育时期的个体（卵、幼虫、蛹和蛾）中均有表达，且表达量趋于一致。表明乳酸脱氢酶基因在野桑蚕的生命活动中可能具有重要的生理功能。
위료연구야상잠유산탈경매기인적공능，선택야상잠5령3일유충적조직급불동발육시기적개체，이용 PCR 기술대야상잠유산탈경매기인진행극륭，득도야상잠유산탈경매기인개방열독광장도위996 bp，몰유내함자，지유1개외현자。해기인편마적단백함유331개안기산잔기，등전점위6·76，분자량위36·35 kD。안기산서렬분석표명，해단백고도보수，아세포정위예측분석표명해단백주요정위우세포질중。전록표체분석표명，해기인재야상잠5령3일유충적조직（마씨관、중장、표피、지방체、사선、혈액、정소화란소）급불동발육시기적개체（란、유충、용화아）중균유표체，차표체량추우일치。표명유산탈경매기인재야상잠적생명활동중가능구유중요적생리공능。
In order to explore the functions of lactate dehydrogenase(LDH),using the different tissues of 3 day of fifth instar larvae and the different developmental stages individuals,the LDH gene from Bombyx mandarina was cloned by polymerase chain reaction.The results showed that the open reading frame of LDH was 996 bp,encoding 331 amino acid,containing 1 exon,no intron,and a predicted pI of 6.76 and molecular weight of 36·35 kD.The deduced amino acid sequence was highly conserved.The predicted analysis showed that the LDH was located in the cytoplasm.Transcriptal expression pattern revealed that the LDH was expressed in the tissues (malpig-hian tubule,midgut,epidermis,fat body,silk gland,blood,testis,ovary ) and developmental stages(egg,larva,pupa,moth),and the expression level was almost consistent. LDH may play an important physiological function in vital movement of Bombyx mandarina .