海峡药学
海峽藥學
해협약학
STRAIT PHARMACEUTICAL JOURNAL
2014年
10期
55-56,57
,共3页
HPLC%湿疹霜%含量测定%盐酸苯海拉明
HPLC%濕疹霜%含量測定%鹽痠苯海拉明
HPLC%습진상%함량측정%염산분해랍명
HPLC%Eczema cream%Diphenhydramine%Hydrochloride%Determination
目的：建立HPLC法测定复方苯海拉明霜中盐酸苯海拉明含量。方法色谱柱：ZORBAX Eclipse-CN（4.6×150mm，5μm）；流动相：乙腈-水-三乙胺（50∶50∶0.5）（用冰醋酸调节pH值至6.5）；检测波长：258nm；流速：0.6mL· min -1；柱温：28℃；进样量：20μL结果盐酸苯海拉明在0.504～20.160μg范围内质量浓度与峰面积线性关系良好（r＝0.9996），平均回收率98.36％，RSD为0.2％。结论所建方法准确、重复性好、可靠，能够控制复方苯海拉明霜的质量。
目的：建立HPLC法測定複方苯海拉明霜中鹽痠苯海拉明含量。方法色譜柱：ZORBAX Eclipse-CN（4.6×150mm，5μm）；流動相：乙腈-水-三乙胺（50∶50∶0.5）（用冰醋痠調節pH值至6.5）；檢測波長：258nm；流速：0.6mL· min -1；柱溫：28℃；進樣量：20μL結果鹽痠苯海拉明在0.504～20.160μg範圍內質量濃度與峰麵積線性關繫良好（r＝0.9996），平均迴收率98.36％，RSD為0.2％。結論所建方法準確、重複性好、可靠，能夠控製複方苯海拉明霜的質量。
목적：건립HPLC법측정복방분해랍명상중염산분해랍명함량。방법색보주：ZORBAX Eclipse-CN（4.6×150mm，5μm）；류동상：을정-수-삼을알（50∶50∶0.5）（용빙작산조절pH치지6.5）；검측파장：258nm；류속：0.6mL· min -1；주온：28℃；진양량：20μL결과염산분해랍명재0.504～20.160μg범위내질량농도여봉면적선성관계량호（r＝0.9996），평균회수솔98.36％，RSD위0.2％。결론소건방법준학、중복성호、가고，능구공제복방분해랍명상적질량。
OBJECTIVE To establish a HPLC method for determinate diphenhydramine hydrochloride content in compound diphenhydramine cream.METHODS The column was Eclipse XDB-CN(4.6 ×150mm,5μm);The mobile phase was acetonitrile-water-triethylamine ( 50∶50∶0.5 ) ( with glacial acetic acid pH adjusted to 6.5 ).The detection wavelength was 258 nm;Flow rate:0.6 mL · min-1;The column temperature was 28℃;The injection vol-ume was 20μL.RESULTS The diphenhydramine hydrochloride had good relationship in 0.504~20.160μg linear within the range of concentration and peak area ( r =0.9996 ) , average yield was 98.36%, RSD was 0.2%.CONCLUSION The method is accurate , reproducible , reliable and can provide a reference for the detection of compound diphenhydramine cream diphenhydramine hydrochloride content.
