海峡药学
海峽藥學
해협약학
STRAIT PHARMACEUTICAL JOURNAL
2014年
10期
46-48
,共3页
HPLC%保健食品%维生素C%含量
HPLC%保健食品%維生素C%含量
HPLC%보건식품%유생소C%함량
Determination%Vitamin C%Health food
目的：建立保健食品中维生素C的高效液相色谱测定方法。方法采用symmetry C18柱分离，以甲醇＋乙酸铵溶液（5＋95）为流动相，流速1.00mL· min -1，二级管阵列检测器，检测波长266nm。结果本方法线性范围为0.01～0.5mg· mL -1，加标回收率在91.7％～103.4％之间，RSD在0.56％～1.81％之间。结论该方法简便、快速、准确，适用于保健食品中维生素C含量的测定。
目的：建立保健食品中維生素C的高效液相色譜測定方法。方法採用symmetry C18柱分離，以甲醇＋乙痠銨溶液（5＋95）為流動相，流速1.00mL· min -1，二級管陣列檢測器，檢測波長266nm。結果本方法線性範圍為0.01～0.5mg· mL -1，加標迴收率在91.7％～103.4％之間，RSD在0.56％～1.81％之間。結論該方法簡便、快速、準確，適用于保健食品中維生素C含量的測定。
목적：건립보건식품중유생소C적고효액상색보측정방법。방법채용symmetry C18주분리，이갑순＋을산안용액（5＋95）위류동상，류속1.00mL· min -1，이급관진렬검측기，검측파장266nm。결과본방법선성범위위0.01～0.5mg· mL -1，가표회수솔재91.7％～103.4％지간，RSD재0.56％～1.81％지간。결론해방법간편、쾌속、준학，괄용우보건식품중유생소C함량적측정。
OBJECTIVE To establish a high performance liquid chromatography ( HPLC ) method to test vita-mins C in health food.METHODS The sepration was carried out on symmetry C 18 column.Methanol-ammonium acetate solution (5+95) were used respectively as mobile phases.The flow rate was 1.0mL· min-1.The detector was PDA.The decetive wavelength was set at 266 nm.RESULTS The calibration curves were liner in the range of 0.01 ~0.5 mg · mL-1 , the rate of recovery were 91.7% to 103.4% with RSD from 1.44% to 4.18%).CONCLUSION The proposed method is simple ,sensitive.Accurate and can be used reliable to test vitamins C in health food.
