CAJ | 학술논문

目的：探讨荧光 VEGF165-USPIO（血管内皮生长因子165-超顺磁性氧化铁）作为 MR 对比剂的可行性，检测该对比剂体外对 VEGF165抗原的靶向性。方法：通过氨基-羧基偶联方式将 VEGF165单克隆抗体和 USPIO 连接，然后将 Cy5．5标记到单克隆抗体表面，构建 Cy5．5-anti-VEGF165-USPIO 靶向对比剂。以离心和磁分离器的洗涤手段进行改良 ELISA 实验检测其体外活性，实验组加入0．25ml 的荧光-anti-VEGF165-USPIO，对照组加入相同量的荧光 USPIO，每组各设5个离心管，两样本为计量资料，采用独立 t 检验比较不同组间吸光值差异有无显著性。结果：通过分光光度计验证对比剂连接成功，VEGF165单克隆抗体偶联率达到了71．7％，CY5．5的偶联率为83．3％。ELISA 实验表明实验组和对照组各自吸光值差异有显著性意义（P ＜0．05），实验组 OD（吸光值）明显高于对照组。结论：荧光 anti-VEGF165-US-PIO 体外有结合 VEGF165的活性，为进一步的 MR 肿瘤靶向对比剂研究奠定了实验基础。
목적：탐토형광 VEGF165-USPIO（혈관내피생장인자165-초순자성양화철）작위 MR 대비제적가행성，검측해대비제체외대 VEGF165항원적파향성。방법：통과안기-최기우련방식장 VEGF165단극륭항체화 USPIO 련접，연후장 Cy5．5표기도단극륭항체표면，구건 Cy5．5-anti-VEGF165-USPIO 파향대비제。이리심화자분리기적세조수단진행개량 ELISA 실험검측기체외활성，실험조가입0．25ml 적형광-anti-VEGF165-USPIO，대조조가입상동량적형광 USPIO，매조각설5개리심관，량양본위계량자료，채용독립 t 검험비교불동조간흡광치차이유무현저성。결과：통과분광광도계험증대비제련접성공，VEGF165단극륭항체우련솔체도료71．7％，CY5．5적우련솔위83．3％。ELISA 실험표명실험조화대조조각자흡광치차이유현저성의의（P ＜0．05），실험조 OD（흡광치）명현고우대조조。결론：형광 anti-VEGF165-US-PIO 체외유결합 VEGF165적활성，위진일보적 MR 종류파향대비제연구전정료실험기출。
Objective:To explore the feasibility of the synthesis of a MR contrast agent Fluorescence VEGF165-US-PIO and to establish an ELISA method for detecting the targeting of this agent to VEGF165-USPIO antigen in vitro. Methods:By applying the coupling of amino-carboxyl,the VEGF165 monoclonal antibody and USPIO were coupled.Then the Cy5.5 was marked to the surfac of monoclonal antibody,and Cy5.5 anti-VEGF165-USPIO targeting contrast agent was established.The centrifuge and magnetic separation were used as the washing solution.Two groups were set up.In the test group,fluorescence anti-VEGF165-USPIO (0.25mL)was used,while in the control group the same amount of fluorescence-USPIO was applied.Each group had five centrifugal pipes.By applying the two-sample independent T-test,we compared the data obtained from both washings.Results:The new type of magnetic resonance contrast agent was successfully combined. Coupling efficiency of VEGF165 monoclonal antibody was 71.7%,and CY5.5 was 83.3%.ELISA assay showed that ab-sorbance values of the test group and the control group were different (P <0.05 ),while the test group (absorbance) showing a significantly higher OD (absorbance ).Conclusion:Fluorescent anti-VEGF165-USPIO has activity to bind VEGF165 in vitro.