中国蔬菜
中國蔬菜
중국소채
CHINA VEGETABLES
2014年
11期
15-19
,共5页
林燕%李菲%张淑江%张慧%章时藩%孙日飞%张振贤
林燕%李菲%張淑江%張慧%章時藩%孫日飛%張振賢
림연%리비%장숙강%장혜%장시번%손일비%장진현
大白菜%小孢子%RNA 提取%转录组测序
大白菜%小孢子%RNA 提取%轉錄組測序
대백채%소포자%RNA 제취%전록조측서
Chinese cabbage%Micro-spore%RNA extraction%Transcriptome sequencing
为了找到适用于转录组测序要求的大白菜小孢子总 RNA 提取方法，以出胚率较高的吉红82为试材，以热激诱导处理后的小孢子为研究对象，比较分析了 TRIzol 法、改良 CTAB 法及超纯试剂盒法3种方法的 RNA 提取效果。结果表明：TRIzol法（磨样）和改良 CTAB 法提取的 RNA 浓度较高，但完整性较差；超纯 RNA 提取试剂盒法提取的 RNA 质量最高，且完整性好，能够满足转录组测序的要求。
為瞭找到適用于轉錄組測序要求的大白菜小孢子總 RNA 提取方法，以齣胚率較高的吉紅82為試材，以熱激誘導處理後的小孢子為研究對象，比較分析瞭 TRIzol 法、改良 CTAB 法及超純試劑盒法3種方法的 RNA 提取效果。結果錶明：TRIzol法（磨樣）和改良 CTAB 法提取的 RNA 濃度較高，但完整性較差；超純 RNA 提取試劑盒法提取的 RNA 質量最高，且完整性好，能夠滿足轉錄組測序的要求。
위료조도괄용우전록조측서요구적대백채소포자총 RNA 제취방법，이출배솔교고적길홍82위시재，이열격유도처리후적소포자위연구대상，비교분석료 TRIzol 법、개량 CTAB 법급초순시제합법3충방법적 RNA 제취효과。결과표명：TRIzol법（마양）화개량 CTAB 법제취적 RNA 농도교고，단완정성교차；초순 RNA 제취시제합법제취적 RNA 질량최고，차완정성호，능구만족전록조측서적요구。
In order to find out micro-spore extracting method suitable for transcriptome sequencing,this study took Chinese cabbage‘Jihong 82’as experimental material and micro-spore induced with heat shock as research object.This study compared and analyzed the RNA extraction effects by 3 different methods:TRIzol method,improved CTAB method and ultrapure kit method.The results showed that RNA concentrations extracted by TRIzol method and improved CTAB method were higher,but the RNA integrity was lower.Quality of RNA extracted by ultrapure kit method was the highest,and the RNA inegrity was also good.Therefore,RNA obtained by this method was suitable for satisfying the requirement of transcriptome sequencing.
