CAJ | 학술논문

利用菁染料超分子作为分子探针对黄腐酸进行标记，检测黄腐酸和药效机制，对于生命科学和药物化学领域的发展具有重要意义。采用紫外-可见吸收光谱和圆二色谱研究黄腐酸作为模板对不同结构菁染料超分子的组装及手性调控。结果表明：黄腐酸能诱导三种菁染料 J-聚集体解聚为单体并伴有体系表观颜色的变化，并与菁染料单体之间具有很高亲和力。黄腐酸作为模板不仅可以诱导M TC形成具有一定手性的H-聚集体，而且能使M TC的 H-聚集体的手性出现两次反转；还可以诱导ETC的J-聚集体发生分子间的重排，最终形成具有左手螺旋性的J-聚集体。除此之外，黄腐酸还对PTC的J-聚集体圆二色谱信号有显著的影响。同时，菁染料中位取代基影响到相互作用的能力：中位取代基越小，菁染料聚集体与黄腐酸的作用越强。菁染料与黄腐酸的结合能力大小为M TC＞ETC＞ PTC。菁染料超分子可作为一类优良的分子探针对黄腐酸进行特异性识别并可达到可视化的检测效果。
이용정염료초분자작위분자탐침대황부산진행표기，검측황부산화약효궤제，대우생명과학화약물화학영역적발전구유중요의의。채용자외-가견흡수광보화원이색보연구황부산작위모판대불동결구정염료초분자적조장급수성조공。결과표명：황부산능유도삼충정염료 J-취집체해취위단체병반유체계표관안색적변화，병여정염료단체지간구유흔고친화력。황부산작위모판불부가이유도M TC형성구유일정수성적H-취집체，이차능사M TC적 H-취집체적수성출현량차반전；환가이유도ETC적J-취집체발생분자간적중배，최종형성구유좌수라선성적J-취집체。제차지외，황부산환대PTC적J-취집체원이색보신호유현저적영향。동시，정염료중위취대기영향도상호작용적능력：중위취대기월소，정염료취집체여황부산적작용월강。정염료여황부산적결합능력대소위M TC＞ETC＞ PTC。정염료초분자가작위일류우량적분자탐침대황부산진행특이성식별병가체도가시화적검측효과。
Using cyanine dyes supramolecular as molecular probes to mark FA has important significance in life sciences and pharmaceutical chemistry ,w hich can detect FA as drug efficacy mechanism and the change in physiological activity .In the pres-ent paper ,we investigated supramolecular assembly and chiral inducement of cyanine dyes template by FA with absorption and circular dichroism (CD) spectra .The result suggests that FA can induce cyanine dyes from J-aggregation to monomer along with different colors change and has strong affinity with cyanine dye monomer .The template of FA not only can translate the chirality of MTC H-aggregation to other two states ,but also induce ETC J-aggregation to molecular rearrangement and form left-handed helix of J-aggregates .Besides ,the association of PTC with FA ,i .e .binding to FA gave rise to the J-aggregation CD signals . Meanwhile ,it was inferred that the meso substituent of cyanine dyes play an important role in the interaction between FA and the J-aggregation:the smaller the meso substituent ,the higher the affinity interacted with FA .Clearly ,the binding abilities be-tween cyanine dyes and FA follow the order of MTC> ETC> PTC .These results support that the cyanine dyes supramolecular aggregates can be used as a kind of excellent molecular probes for specific recognition of FA and achieve the effect of visual in-spection .