CAJ | 학술논문

利用红外光谱三级鉴定方法对藏药川西獐牙菜及其不同溶液提取物进行分析研究。选择川西獐牙菜中含量相对较高的药用成分龙胆苦苷作为不同溶液提取物的对照成分进行红外光谱和二阶导数光谱的分析；同时对不同浓度乙醇提取物进行二维相关光谱分析。结果显示，龙胆苦苷红外光谱中1611和1075 cm -1处的两个主要特征峰强度的变化趋势，和川西獐牙菜不同溶剂提取物中龙胆苦苷含量变化趋势一致；提取溶剂极性相近时，提取物的红外光谱图相似；随着提取溶液极性的逐渐降低，提取物的红外谱图中2853，1733，1464，1277和1161 cm-1等处吸收峰强度逐渐增强，提取物中酯类化合物含量逐渐增加，萜类化合物的提取率逐渐增加；红外光谱图极为相近的不同浓度乙醇提取物在二维红外光谱图中有很明显的差异。龙胆苦苷特征峰强度的变化趋势和不同提取物中龙胆苦苷含量变化趋势的一致性说明红外光谱分析结果能体现分析对象中所含有成分的含量变化；川西獐牙菜原药材，水提物和不同浓度乙醇提取物谱图的相似性和相似度的变化规律，说明传统用药水煎液和低浓度醇提物的科学性；红外光谱技术可成为川西獐牙菜不同提取物后期药理活性成分宏观控制，和多以复方入药的藏药研究中准确、快速、有效的分析方法。
이용홍외광보삼급감정방법대장약천서장아채급기불동용액제취물진행분석연구。선택천서장아채중함량상대교고적약용성분룡담고감작위불동용액제취물적대조성분진행홍외광보화이계도수광보적분석；동시대불동농도을순제취물진행이유상관광보분석。결과현시，룡담고감홍외광보중1611화1075 cm -1처적량개주요특정봉강도적변화추세，화천서장아채불동용제제취물중룡담고감함량변화추세일치；제취용제겁성상근시，제취물적홍외광보도상사；수착제취용액겁성적축점강저，제취물적홍외보도중2853，1733，1464，1277화1161 cm-1등처흡수봉강도축점증강，제취물중지류화합물함량축점증가，첩류화합물적제취솔축점증가；홍외광보도겁위상근적불동농도을순제취물재이유홍외광보도중유흔명현적차이。룡담고감특정봉강도적변화추세화불동제취물중룡담고감함량변화추세적일치성설명홍외광보분석결과능체현분석대상중소함유성분적함량변화；천서장아채원약재，수제물화불동농도을순제취물보도적상사성화상사도적변화규률，설명전통용약수전액화저농도순제물적과학성；홍외광보기술가성위천서장아채불동제취물후기약리활성성분굉관공제，화다이복방입약적장약연구중준학、쾌속、유효적분석방법。
The objective of the present study is to research the herb of Swertia mussotii Franch and its different extracts by tri-step infrared spectroscopy .The main constitute of Swertia mussotii Franch-gentiamarin ,which is also the higher content consti-tute ,was selected as the control components to analyze the infrared spectroscopy and second derivative infrared spectroscopy of different extracts of Swertia mussotii Franch ,at the same time ,the different concentration of ethanol extracts were also analyzed by two-dimensional correlation spectroscopy(2D-COS) .The results indicated that the intensity of 1 611 and 1 075 cm -1 of gen-tiamarin ,which are its two main absorptions in the infrared spectra ,has the positive correlation with the content change in dif-ferent extracts .The infrared spectroscopy of extracts are similar if the polarity of extract solvents is close;with the decreases in solution polarity ,the intensity of 2 853 ,1 733 ,1 464 ,1 277 and 1 161 cm-1 in infrared spectroscopy of different extracts is in-creased ,the content of esters and the extraction percentage terpenoid compounds are also increased ;the different concentration of ethanol extracts has obviously difference when they are analyzed by two - dimensional correlation spectroscopy(2D-COS) . The positive correlation between the intensity of absorptions and the content of the gentiamarin indicates that the infrared spec-troscopy can reflect the content change in constitute;the similar and the change trend of the different concentrations of ethanol extract infrared spectroscopy approve the scientificalness of decoction of traditional medicine;infrared spectroscopy that used in the research can be used as an accurate ,rapid and effective method in the pharmacological activity tests of transitional herbal Swertia mussotii F .and it’s different extracts ,even in the research on the tibetan medicine .