光谱学与光谱分析
光譜學與光譜分析
광보학여광보분석
SPECTROSCOPY AND SPECTRAL ANALYSIS
2014年
11期
2923-2928
,共6页
高丽丽%王盛锋%韩亚%刘自飞%黄金生%Hilman%刘荣乐%汪洪
高麗麗%王盛鋒%韓亞%劉自飛%黃金生%Hilman%劉榮樂%汪洪
고려려%왕성봉%한아%류자비%황금생%Hilman%류영악%왕홍
花生%傅里叶红外光谱(FTIR)%缺钙
花生%傅裏葉紅外光譜(FTIR)%缺鈣
화생%부리협홍외광보(FTIR)%결개
Peanut%Fourier transform infrared spectrometry (FTIR)%Calcium deficiency
钙（Ca）是植物必需营养元素之一，对植物的生长、发育与生理代谢等起着重要作用。缺钙会减少作物的产量，降低品质，因此影响经济价值。花生（A rachis hy pogaea）是对缺钙很敏感的作物。试验选取L H11与YZ9102两个花生品种为供试作物，待花生幼苗长出第一片真叶以后，转移至营养液中，分别在三个钙处理（0，0.01和2.0 mmol · L -1）下培养28 d ，随后取样分析花生幼苗生长情况与钙吸收，并利用傅里叶红外光谱（FTIR）检测花生苗期植株不同器官的特征吸收峰，研究缺钙胁迫下花生苗期植株体内的化学组分变化。试验结果表明：在0和0.01 mmol · L -1钙处理下，花生品种YZ9102并未出现缺钙症状，但是品种L H11缺钙症状明显：叶片变小、植株变矮、茎尖顶端生长点坏死，基部侧枝数增多。品种YZ9102植株体内与L H11相比较，有较高的钙浓度与钙积累量。0和0.01 mmol · L -1的钙缺钙营养液培养下，品种L H11根系钙积累量在整个植株中所占比例较高，而品种YZ9102在叶中钙积累比例较高。与正常钙供应处理相比，缺钙胁迫下，品种LH11根、茎、叶FTIR谱在1060，1380，1655，2922和3420 cm -1波长附近透射率较高，表明缺钙胁迫下花生幼苗植株体内蛋白质、糖类和脂类等组分明显降低，但品种YZ9102受到缺钙胁迫影响较小。品种YZ9102比L H11更耐缺钙胁迫。
鈣（Ca）是植物必需營養元素之一，對植物的生長、髮育與生理代謝等起著重要作用。缺鈣會減少作物的產量，降低品質，因此影響經濟價值。花生（A rachis hy pogaea）是對缺鈣很敏感的作物。試驗選取L H11與YZ9102兩箇花生品種為供試作物，待花生幼苗長齣第一片真葉以後，轉移至營養液中，分彆在三箇鈣處理（0，0.01和2.0 mmol · L -1）下培養28 d ，隨後取樣分析花生幼苗生長情況與鈣吸收，併利用傅裏葉紅外光譜（FTIR）檢測花生苗期植株不同器官的特徵吸收峰，研究缺鈣脅迫下花生苗期植株體內的化學組分變化。試驗結果錶明：在0和0.01 mmol · L -1鈣處理下，花生品種YZ9102併未齣現缺鈣癥狀，但是品種L H11缺鈣癥狀明顯：葉片變小、植株變矮、莖尖頂耑生長點壞死，基部側枝數增多。品種YZ9102植株體內與L H11相比較，有較高的鈣濃度與鈣積纍量。0和0.01 mmol · L -1的鈣缺鈣營養液培養下，品種L H11根繫鈣積纍量在整箇植株中所佔比例較高，而品種YZ9102在葉中鈣積纍比例較高。與正常鈣供應處理相比，缺鈣脅迫下，品種LH11根、莖、葉FTIR譜在1060，1380，1655，2922和3420 cm -1波長附近透射率較高，錶明缺鈣脅迫下花生幼苗植株體內蛋白質、糖類和脂類等組分明顯降低，但品種YZ9102受到缺鈣脅迫影響較小。品種YZ9102比L H11更耐缺鈣脅迫。
개（Ca）시식물필수영양원소지일，대식물적생장、발육여생리대사등기착중요작용。결개회감소작물적산량，강저품질，인차영향경제개치。화생（A rachis hy pogaea）시대결개흔민감적작물。시험선취L H11여YZ9102량개화생품충위공시작물，대화생유묘장출제일편진협이후，전이지영양액중，분별재삼개개처리（0，0.01화2.0 mmol · L -1）하배양28 d ，수후취양분석화생유묘생장정황여개흡수，병이용부리협홍외광보（FTIR）검측화생묘기식주불동기관적특정흡수봉，연구결개협박하화생묘기식주체내적화학조분변화。시험결과표명：재0화0.01 mmol · L -1개처리하，화생품충YZ9102병미출현결개증상，단시품충L H11결개증상명현：협편변소、식주변왜、경첨정단생장점배사，기부측지수증다。품충YZ9102식주체내여L H11상비교，유교고적개농도여개적루량。0화0.01 mmol · L -1적개결개영양액배양하，품충L H11근계개적루량재정개식주중소점비례교고，이품충YZ9102재협중개적루비례교고。여정상개공응처리상비，결개협박하，품충LH11근、경、협FTIR보재1060，1380，1655，2922화3420 cm -1파장부근투사솔교고，표명결개협박하화생유묘식주체내단백질、당류화지류등조분명현강저，단품충YZ9102수도결개협박영향교소。품충YZ9102비L H11경내결개협박。
The objective of the present study was to reveal different tolerance of peanut plants to Ca deficiency by determining Ca uptake and Fourier transform infrared spectral (FTIR) differences of two peanut cultivars grown in nutrition solution .Peanut cultivars LH11 and YZ9102 were selected .Seedlings at the first leaf stage were cultivated for 28 days in nutrient solution with 0 , 0.01 and 2.0 mmol · L -1 Ca treatments ,respectively .The results showed that under 0 and 0.01 mmol · L -1 Ca supply , YZ9102 did not show Ca deficiency symptoms and the plant biomass did not change ,whereas LH11 exhibited shoot-tip necrosis , smaller plant size ,more lateral branches ,and plant dry matter weights decreased significantly .YZ9102 had higher plant Ca con-centration and Ca accumulation than LH11 .Besides ,for LH11 ,Ca was mainly accumulated in roots ,while for YZ9102 mainly in leaves .As compared with plants cultivated in 2.0 mol · L -1 Ca nutrition ,root ,stem and leaf of LH11 plants under Ca defi-ciency stress showed higher transmittance at peaks 1 060 ,1 380 ,1 655 ,2 922 ,and 3 420 cm-1 in FTIR spectra ,indicating that the contents of protein ,sugar and lipid decreased obviously in LH11 plants in condition that Ca supply was limited .However , the FTIR spectra of YZ9102 were less affected by Ca deficiency .It is suggested that YZ9102 might be more tolerant to Ca defi-ciency .
