中国药业
中國藥業
중국약업
CHINA PHARMACEUTICALS
2014年
20期
63-64
,共2页
升阳十一味丸%高效液相色谱法%没食子酸
升暘十一味汍%高效液相色譜法%沒食子痠
승양십일미환%고효액상색보법%몰식자산
Shengyang Shiyiwei Pills%HPLC%gallic acid
目的：建立测定蒙成药升阳十一味丸中没食子酸含量的高效液相色谱法。方法色谱柱为C18柱（250 mm ×4.6 mm，5μm），流动相为甲醇-0.1%磷酸水（5：95），检测波长为273 nm，流速为1.0 mL/min，柱温为30℃。结果没食子酸进样量在54.04~540.4 ng范围内与峰面积积分值线性关系良好（ r=1.0000），回归方程为 Y=12095 X-797509；平均加样回收率为99.50%，RSD为1.73%（ n=9）。结论该方法准确、精密、重复性好，可用于升阳十一味丸中没食子酸的含量测定。
目的：建立測定矇成藥升暘十一味汍中沒食子痠含量的高效液相色譜法。方法色譜柱為C18柱（250 mm ×4.6 mm，5μm），流動相為甲醇-0.1%燐痠水（5：95），檢測波長為273 nm，流速為1.0 mL/min，柱溫為30℃。結果沒食子痠進樣量在54.04~540.4 ng範圍內與峰麵積積分值線性關繫良好（ r=1.0000），迴歸方程為 Y=12095 X-797509；平均加樣迴收率為99.50%，RSD為1.73%（ n=9）。結論該方法準確、精密、重複性好，可用于升暘十一味汍中沒食子痠的含量測定。
목적：건립측정몽성약승양십일미환중몰식자산함량적고효액상색보법。방법색보주위C18주（250 mm ×4.6 mm，5μm），류동상위갑순-0.1%린산수（5：95），검측파장위273 nm，류속위1.0 mL/min，주온위30℃。결과몰식자산진양량재54.04~540.4 ng범위내여봉면적적분치선성관계량호（ r=1.0000），회귀방정위 Y=12095 X-797509；평균가양회수솔위99.50%，RSD위1.73%（ n=9）。결론해방법준학、정밀、중복성호，가용우승양십일미환중몰식자산적함량측정。
Objective To establish the high performance liquid chromatography ( HPLC ) method of the gallic acid content in Mongolian patent medicine Shengyang Shiyiwei Pills. Methods The chromatographic separation was carried out on the Phenomen C18 column (250 mm × 4. 6 mm,5 μm)with methanol-0. 1% phosphoric acid(5 :95)as the mobile phase;the detection wavelength was set at 273 nm;the flow rate was 1. 0 mL/min and the column temperature was 30 ℃. Results The sample size of gallic acid in the concentration ranges of 54. 04-540. 4 ng showed good linear relation with the peak area ( r=1. 000 0),the regression equation was Y=12 095 X-797 509;the average recovery rate was 99. 50%,RSD was 1. 73%( n=9). Conclusion The method is accurate and precise with good reproducibility,which can be used as the determination method of the gallic acid content in Mongolian patent medicine Shengyang Shiyiwei Pills.
