中国药业
中國藥業
중국약업
CHINA PHARMACEUTICALS
2014年
20期
61-62
,共2页
王建平%杨云%张兰欣%惠金玲%马长勇%张学慧%迟平%刘茵%张娟%颜耀东
王建平%楊雲%張蘭訢%惠金玲%馬長勇%張學慧%遲平%劉茵%張娟%顏耀東
왕건평%양운%장란흔%혜금령%마장용%장학혜%지평%류인%장연%안요동
儿茶素%鞣质%缩合鞣质%香草醛硫酸法%紫外分光光度法%榆黄喷雾剂%榆树皮
兒茶素%鞣質%縮閤鞣質%香草醛硫痠法%紫外分光光度法%榆黃噴霧劑%榆樹皮
인다소%유질%축합유질%향초철류산법%자외분광광도법%유황분무제%유수피
catechin%condensed tannins%vanillin-H2SO4 method%UV spectrophotometry%Yuhuang Spray%elm bark
目的：建立测定榆黄喷雾剂中缩合鞣质含量的方法。方法供试品溶液中依次加入3%香草醛甲醇溶液、30%浓硫酸甲醇溶液各5 mL后避光显色20 min，用紫外分光光度法在500 nm波长处测定吸光度并计算样品中缩合鞣质含量。结果儿茶素对照品质量浓度在0.03~0.3 g/L 范围内与吸光度呈良好线性关系，回归方程为 Y=3.0652 X+0.0257，r=0.9993（ n=10）；加样回收率为99.62%，RSD为1.76%（ n=6）。结论香草醛硫酸法测定缩合鞣质含量，方法简便、快速、精密度、稳定性、重复性、专属性好，适合作为榆黄喷雾剂中缩合鞣质的含量检测方法。
目的：建立測定榆黃噴霧劑中縮閤鞣質含量的方法。方法供試品溶液中依次加入3%香草醛甲醇溶液、30%濃硫痠甲醇溶液各5 mL後避光顯色20 min，用紫外分光光度法在500 nm波長處測定吸光度併計算樣品中縮閤鞣質含量。結果兒茶素對照品質量濃度在0.03~0.3 g/L 範圍內與吸光度呈良好線性關繫，迴歸方程為 Y=3.0652 X+0.0257，r=0.9993（ n=10）；加樣迴收率為99.62%，RSD為1.76%（ n=6）。結論香草醛硫痠法測定縮閤鞣質含量，方法簡便、快速、精密度、穩定性、重複性、專屬性好，適閤作為榆黃噴霧劑中縮閤鞣質的含量檢測方法。
목적：건립측정유황분무제중축합유질함량적방법。방법공시품용액중의차가입3%향초철갑순용액、30%농류산갑순용액각5 mL후피광현색20 min，용자외분광광도법재500 nm파장처측정흡광도병계산양품중축합유질함량。결과인다소대조품질량농도재0.03~0.3 g/L 범위내여흡광도정량호선성관계，회귀방정위 Y=3.0652 X+0.0257，r=0.9993（ n=10）；가양회수솔위99.62%，RSD위1.76%（ n=6）。결론향초철류산법측정축합유질함량，방법간편、쾌속、정밀도、은정성、중복성、전속성호，괄합작위유황분무제중축합유질적함량검측방법。
Objective To establish the quantitative determination method of condensed tannins in Yuhuang Spray. Methods The test so-lution was added with 3% vanillin methanol solution 5 mL and 30% concentrated sulfuric acid methanol solution 5 mL in turn,then which was developed by keeping away from light for 20 min,the absorbance was detected at 500 nm by spectrophotometry and the condensed tannin content in the sample was calculated. Results The concentration of catechins reference substance had a good linear relationship with the absorbance in the range of 0. 03-0. 3 g/L,and the regression equation was Y=3. 065 2 X+0. 025 7,r=0. 999 3 ( n=10 ) ,the average recovery rate was 99. 62%,RSD=1. 76%( n=6). Conclusion The vanillin-H2SO4 method is simple and rapid with good precision,stability,reproducibility and specificity and suitable for the determination of the condensed tannins content in Yuhuang Spray.
