CAJ | 학술논문

目的：观察质粒mir-129对肾癌细胞SN12-PM6生长曲线的影响。方法质粒mir-129介导下转染肾癌细胞SN12-PM6，以未转染质粒mir-129肾癌细胞SN12-PM6作为对照组，以转染的脂质体2000质粒mir-129肾癌细胞SN12-PM6作为实验组，MTT法测定细胞OD（570nm）值，绘制两组肾癌细胞生长曲线。结果肾癌细胞SN12-PM6转染质粒mir-129，其生长明显受到抑制，P＜0．05，两者统计学上有显著性差异。结论转染质粒mir-129能明显抑制肾癌细胞SN12-PM6生长。
목적：관찰질립mir-129대신암세포SN12-PM6생장곡선적영향。방법질립mir-129개도하전염신암세포SN12-PM6，이미전염질립mir-129신암세포SN12-PM6작위대조조，이전염적지질체2000질립mir-129신암세포SN12-PM6작위실험조，MTT법측정세포OD（570nm）치，회제량조신암세포생장곡선。결과신암세포SN12-PM6전염질립mir-129，기생장명현수도억제，P＜0．05，량자통계학상유현저성차이。결론전염질립mir-129능명현억제신암세포SN12-PM6생장。
Objective To observe the effect of plasmid Mir-129 on the growth curve of renal cell carcinoma SN12-PM6 cells.Methods Plasmid Mir-129 were transfected with renal cell carcinoma SN 12-PM6 cells without transfection of plasmid mir-129, in renal cell carcinoma SN12-PM6 cells as control group, transfected with Lipofectamine 2000 plasmid mir-129 of renal cell carcinoma cell line SN 12-PM6 as the experimental group .The OD value of MTT method for determination of renal carcinoma cells and draw the growth curves of two groups .Results Renal cell carcinoma SN12-PM6 cells transfected with plasmid mir-129, the growth was inhibited ,P<0.05, there is significant difference statistically .Conclusions Transfection of plasmid mir-129 could obviously inhibit the growth of SN 12-PM6 renal cell carcinoma .