国际检验医学杂志
國際檢驗醫學雜誌
국제검험의학잡지
INTERNATIONAL JOURNAL OF LABORATORY MEDICINE
2014年
20期
2811-2812,2815
,共3页
朱红艳%毕胜%杨曦%李峥%徐永敏
硃紅豔%畢勝%楊晞%李崢%徐永敏
주홍염%필성%양희%리쟁%서영민
丙型肝炎病毒抗体%丙型肝炎病毒 RNA%酶联免疫吸附试验%实时荧光定量 PCR
丙型肝炎病毒抗體%丙型肝炎病毒 RNA%酶聯免疫吸附試驗%實時熒光定量 PCR
병형간염병독항체%병형간염병독 RNA%매련면역흡부시험%실시형광정량 PCR
hepatitis C virus antibodies%hepatitis C virus RNA%enzyme-linked immunosorbent assay%real-time PCR
目的:探讨丙型肝炎病毒核酸和抗体检测方法在人群筛查中的应用。方法采用胶体金快速试验法和酶联免疫吸附试验(ELISA)检测丙型肝炎病毒(HCV)抗体,实时荧光定量 PCR(RT-PCR)检测 HCV-RNA 病毒载量。结果(1)539份样本中,其中266例抗体阴性,263例抗体阳性。(2)在67例 HCV-RNA 病毒载量小于103 IU/mL 组中,ELISA 法检测 HCV 抗体阳性有60例,胶体金快速试验法检测抗体阳性有30例。208例 HCV-RNA 病毒载量大于或等于103 IU/mL 组中,ELISA 法检测的抗体阳性有199例,胶体金快速法检测阳性的有181例,另外有6例两种抗体检测均为阴性患者 RNA 病毒载量大于或等于103 IU/mL。(3)对208例 HCV-RNA 病毒载量大于或等于103 IU/mL 样本分成4个组。GGT、ALT 及 AST 在4组间差异均有统计学意义(P <0.05),而 ALB 及 S/CO 值在4组间差异无统计学意义(P >0.05)。结论在人群筛查中为了减少漏诊率,尽早诊断丙型肝炎,要联合运用以上各实验室检测方法,综合分析。
目的:探討丙型肝炎病毒覈痠和抗體檢測方法在人群篩查中的應用。方法採用膠體金快速試驗法和酶聯免疫吸附試驗(ELISA)檢測丙型肝炎病毒(HCV)抗體,實時熒光定量 PCR(RT-PCR)檢測 HCV-RNA 病毒載量。結果(1)539份樣本中,其中266例抗體陰性,263例抗體暘性。(2)在67例 HCV-RNA 病毒載量小于103 IU/mL 組中,ELISA 法檢測 HCV 抗體暘性有60例,膠體金快速試驗法檢測抗體暘性有30例。208例 HCV-RNA 病毒載量大于或等于103 IU/mL 組中,ELISA 法檢測的抗體暘性有199例,膠體金快速法檢測暘性的有181例,另外有6例兩種抗體檢測均為陰性患者 RNA 病毒載量大于或等于103 IU/mL。(3)對208例 HCV-RNA 病毒載量大于或等于103 IU/mL 樣本分成4箇組。GGT、ALT 及 AST 在4組間差異均有統計學意義(P <0.05),而 ALB 及 S/CO 值在4組間差異無統計學意義(P >0.05)。結論在人群篩查中為瞭減少漏診率,儘早診斷丙型肝炎,要聯閤運用以上各實驗室檢測方法,綜閤分析。
목적:탐토병형간염병독핵산화항체검측방법재인군사사중적응용。방법채용효체금쾌속시험법화매련면역흡부시험(ELISA)검측병형간염병독(HCV)항체,실시형광정량 PCR(RT-PCR)검측 HCV-RNA 병독재량。결과(1)539빈양본중,기중266례항체음성,263례항체양성。(2)재67례 HCV-RNA 병독재량소우103 IU/mL 조중,ELISA 법검측 HCV 항체양성유60례,효체금쾌속시험법검측항체양성유30례。208례 HCV-RNA 병독재량대우혹등우103 IU/mL 조중,ELISA 법검측적항체양성유199례,효체금쾌속법검측양성적유181례,령외유6례량충항체검측균위음성환자 RNA 병독재량대우혹등우103 IU/mL。(3)대208례 HCV-RNA 병독재량대우혹등우103 IU/mL 양본분성4개조。GGT、ALT 급 AST 재4조간차이균유통계학의의(P <0.05),이 ALB 급 S/CO 치재4조간차이무통계학의의(P >0.05)。결론재인군사사중위료감소루진솔,진조진단병형간염,요연합운용이상각실험실검측방법,종합분석。
Objective To investigate the application of hepatitis C virus RNA and antibody detection method in population screening.Methods The colloidal gold rapid test method and the enzyme-linked immunosorbent assay (ELISA)were adopted to detect hepatitis C virus (HCV)antibodies,and the real-time quantitative PCR (RT-PCR)was adopted to detect HCV-RNA viral load.Results (1)Among 539 samples,266 cases were antibody negative and 263 cases were antibody positive.(2)Among 67 cases in the HCV-RNA viral load <103 IU/mL group,60 cases were HCV antibody positive by ELISA and 30 cases were HCV antibody positive by colloidal gold rapid test.Among 208 cases in the HCV-RNA viral load ≥ 103 IU/mL,199 cases were antibody positive by ELISA,but only 181cases were antibody positive by the colloidal gold rapid method.Other 6 cases of were 2 kinds of antibody negative had the HCV-RNA viral load ≥ 103 IU/mL.(3)208 cases of HCV-RNA viral load ≥ 103 IU/mL sample were divided in-to four groups.GGT,ALT and AST were statistically significantly different P <0.05),while ALB and S/CO values hadno statisti-cal difference (P >0.05).Conclusion In order to reduce the missed diagnosis rate and diagnose hepatitis C as early as possible,the above laboratory detection methods should be jointly applied and the comprehensive analysis should be conducted in population screening.