CAJ | 학술논문

目的：探讨高血压脑出血患者脑脊液中游离DNA的动态变化与组织损伤的关系。方法54例脑出血患者按照格拉斯哥昏迷分级（GCS）评分分为轻度17例，中度21例和重度16例，分别采集发病后1、3、7、15 d 脑脊液2 mL，提取游离 DNA，采用荧光定量 PCR 技术检测游离 DNA 水平。结果脑出血组中游离 DNA 水平重度组显著高于轻度组和中度组；颅内压异常组高于正常组；颅内感染组高于非感染组。结论脑脊液游离 DNA 水平对于诊断脑出血颅脑损伤有一定诊断价值，而且有助于监控术后并发症的发生。
목적：탐토고혈압뇌출혈환자뇌척액중유리DNA적동태변화여조직손상적관계。방법54례뇌출혈환자안조격랍사가혼미분급（GCS）평분분위경도17례，중도21례화중도16례，분별채집발병후1、3、7、15 d 뇌척액2 mL，제취유리 DNA，채용형광정량 PCR 기술검측유리 DNA 수평。결과뇌출혈조중유리 DNA 수평중도조현저고우경도조화중도조；로내압이상조고우정상조；로내감염조고우비감염조。결론뇌척액유리 DNA 수평대우진단뇌출혈로뇌손상유일정진단개치，이차유조우감공술후병발증적발생。
Objective To study the relationship between the dynamic change of free DNA in cerebrospinal fluid (CSF)and the tissue injury in the patients with hypertensive intracerebral hemorrhage.Methods 54 cases of hypertensive intracerebral hemor-rhage were divided into 3 groups according to the glasgow coma scale(including 17 mild cases,21 moderate cases and 16 severe ca-ses).2 mL of CSF was collected for extracting free DNA on 1,3,7,15 d after stroke onset.The free DNA level was measured by the fluorescent real-time PCR.Results The free DNA level in the severe group was significantly higher than that in the mild and mod-erate groups.The free DNA level in the abnormal intracranial pressure group was higher than that in the normal intracranial pres-sure group;the intracranial infection group was higher than the non-infection group.Conclusion The free DNA level has certain value for diagnosing the craniocerebral injury in hypertensive intracerebral hemorrhage and is conducive to monitor the occurrence of postoperative complications.