CAJ | 학술논문

目的：了解佛山地区淋球菌对5种抗菌药物的耐药性，并检测相关耐药基因突变情况。方法收集57株淋球菌，药敏试验采用琼脂稀释法。PCR 法扩增相关耐药基因，PCR 产物测序结果在 GenBank 中用 blastn 进行核酸序列同源性搜索。结果淋球菌对青霉素、四环素、环丙沙星、头孢曲松、大观霉素的敏感率分别为0．0％、8．8％、7．0％、61．4％、100．0％，产β-内酰胺酶淋球菌和质粒介导的四环素高度耐药淋球菌阳性率分别为35．1％、56．1％。耐药基因突变率均在80％以上。结论头孢曲松、大观霉素可以作为佛山地区治疗淋球菌的首选药物。淋球菌耐药机制较复杂，应加强对相关耐药基因分子流行病学监测。
목적：료해불산지구림구균대5충항균약물적내약성，병검측상관내약기인돌변정황。방법수집57주림구균，약민시험채용경지희석법。PCR 법확증상관내약기인，PCR 산물측서결과재 GenBank 중용 blastn 진행핵산서렬동원성수색。결과림구균대청매소、사배소、배병사성、두포곡송、대관매소적민감솔분별위0．0％、8．8％、7．0％、61．4％、100．0％，산β-내선알매림구균화질립개도적사배소고도내약림구균양성솔분별위35．1％、56．1％。내약기인돌변솔균재80％이상。결론두포곡송、대관매소가이작위불산지구치료림구균적수선약물。림구균내약궤제교복잡，응가강대상관내약기인분자류행병학감측。
Objective To understand the drug resistance of Neisseria gonorrhoeae in Foshan area and to detect the related drug resistant gene mutation situation.Methods 57 strains of Neisseria gonorrhoeae were collected.The drug susceptibility test was per-formed by adopting the agar dilution method.The related drug resistance genes were amplified by PCR and the PCR product se-quencing results were performed the homological searching in GenBank by the BLAST algorithm.Results The sensitive rates of Neisseria gonorrhoeae to penicillin,tetracycline,ciprooxacin,ceftriaxone and spectinomycin were 0.0%,8.8%,7.0%,61.4% and 100.0%,respectively.The rates of beta lactamase-producing Neisseria gonorrhoeae and tetracycline resistant Neisseria gonorrhoeae were 35.1% and 56.1%,respectively.The mutation rate of drug resistance genes were over 80%.Conclusion Ceftriaxone and spectinomycin can be used as the first choice drug for the treatment of Neisseria gonorrhoeae in Foshan region.The drug resistance mechanism of Neisseria gonorrhoeae is complex.The epidemiological monitoring of the Neisseria gonorrhoeae related drug resist-ance genes should be strengthened.