CAJ | 학술논문

聚氨酯原料如二异氰酸酯的皮肤致敏性已有大量报道,而聚氨酯材料本身的致敏研究的报道却很少。采用豚鼠最大剂量实验(ISO 10993-10)研究了3种用于人工血管的聚氨酯材料的迟发型超敏反应。PCUL2为赖氨酸扩链的聚碳酸酯聚氨酯,PCUL2-PO和PCUL2-PN分别为接枝聚乙二醇二缩水甘油醚以及接枝聚乙二醇二胺的梳形聚氨酯。3种材料均为多孔材料,且含有可反应的官能团,便于进一步接枝生物分子以提高血管的原位内皮化。测试组为(70±2)℃浸提(24±2)h 的生理盐水浸提液(0.1 g/mL),生理盐水为阴性对照组,二硝基氯苯为阳性对照组。结果显示,3种受试材料致敏率很低(PCUL2-PO∶2/12；其余1/12)。3种聚氨酯的迟发型超敏反应的结果均为阴性,值得进一步进行体内研究。
취안지원료여이이청산지적피부치민성이유대량보도,이취안지재료본신적치민연구적보도각흔소。채용돈서최대제량실험(ISO 10993-10)연구료3충용우인공혈관적취안지재료적지발형초민반응。PCUL2위뢰안산확련적취탄산지취안지,PCUL2-PO화PCUL2-PN분별위접지취을이순이축수감유미이급접지취을이순이알적소형취안지。3충재료균위다공재료,차함유가반응적관능단,편우진일보접지생물분자이제고혈관적원위내피화。측시조위(70±2)℃침제(24±2)h 적생리염수침제액(0.1 g/mL),생리염수위음성대조조,이초기록분위양성대조조。결과현시,3충수시재료치민솔흔저(PCUL2-PO∶2/12；기여1/12)。3충취안지적지발형초민반응적결과균위음성,치득진일보진행체내연구。
Dermal sensitization resulting from the raw materials of polyurethanes like diisocyanates has been fre-quently reported,however the study on allergic reactions to polyurethanes themselves was seldom seen.In this study,the delayed hypersensitivity to three polyurethanes for vascular grafts has been investigated according to guinea pig maximization test (ISO 10993-10).Among the three materials,PCUL2 was a polycarbonate ure-thane chain-extended with lysine while PCUL2-PO and PCUL2-PN were comb-like polymers from PCUL2 backbones grafted with polyethylene glycol (PEG)diglycidyl ether and PEG bisamine respectively.These mate-rials were highly porous with functional groups intended for conj ugating with bioactive molecules to induce in si-tu endothelialization.The test groups were saline extracts prepared at (70±2)℃for (24±2)h using the maxi-mum extraction concentration (0.1 mg/mL).The saline was used as negative control while the 2,4-dinitrochlo-robenzene in ethanol (1 g/L)was used as positive control.The results showed that the test groups induced mi-nor hypersensitivity (2/12 for PCUL2-PO;1/12 for the others).The negative results of the delayed hypersen-sitivity to the tested polyurethanes suggest that further in vivo studies are worthwhile.