CAJ | 학술논문

采用后嫁接法在溶剂热条件下对新型块体Si O 2大孔材料进行环氧基化改性,以环氧基功能化Si O 2大孔材料作为载体,通过共价结合法固定化诺维信(Novozymes)工业级漆酶,对固定化条件进行了优化,研究了固定化酶与游离酶的酶学性质。结果表明,在固定化时间为4 h、pH 值为4.5、初始酶液浓度为25 mg/mL时,固定化效果最好,固定化漆酶活力达到101.7 U/g；固定化漆酶的最适 pH 值为4.0,最适温度为50℃,其pH 值稳定性和热稳定性都显著优于游离漆酶。固定化漆酶具有可重复操作的性质,与底物反应反复操作10批次后剩余活性为43.4%。
채용후가접법재용제열조건하대신형괴체Si O 2대공재료진행배양기화개성,이배양기공능화Si O 2대공재료작위재체,통과공개결합법고정화낙유신(Novozymes)공업급칠매,대고정화조건진행료우화,연구료고정화매여유리매적매학성질。결과표명,재고정화시간위4 h、pH 치위4.5、초시매액농도위25 mg/mL시,고정화효과최호,고정화칠매활력체도101.7 U/g；고정화칠매적최괄 pH 치위4.0,최괄온도위50℃,기pH 치은정성화열은정성도현저우우유리칠매。고정화칠매구유가중복조작적성질,여저물반응반복조작10비차후잉여활성위43.4%。
A novel macroporous silica block material was epoxy-modified via post-grafting method under solvo-thermal conditions.The epoxy-functionalized macroporous silica were employed as the supports for immobiliza-tion of the commercial Novozymes laccase by using covalent binding method.Immobilization conditions were optimized,the properties of immobilized and free laccase were studied.The optimum conditions for immobiliza-tion of laccase were at the immobilization time of 4 h,the buffer of pH 4.5 and the initial laccase concentration of 25 mg/mL,under these conditions,the activity of immobilized laccase reaches 101.7 U/g.The optimum pH and temperature of immobilized laccase were 4.0 and 50 ℃,respectively.The experiments show that the im-mobilization significantly improves the pH stability and thermal stability of laccase.The immobilized laccase can be used repeatedly,and the remained activity was 43.4% after 10 times of reaction with the substrate.