CAJ | 학술논문

目的：建立心肌细胞缺血再灌注模型，有助于在细胞层面对缺血再灌注损伤的保护机制进行探讨，防治临床的无复流现象。方法：分离并纯化乳鼠心肌细胞，培养心肌细胞至完全同步化。以高纯氮气和纯氧分别模拟缺血液和再灌注液。预设系列缺血时间和再灌注时间，测定细胞活力指标制作时间-细胞存活率曲线图，寻求最佳缺血再灌注时间窗。结果：心肌细胞培养第5天搏动呈同步化，频率约90次/ min。与对照组相比，缺血5 h 损伤组与其他组的差异有统计学意义(P<0.05)。与对照组相比，再灌注2、4和6 h 心肌细胞存活率的下降都有统计学差异，P 值分别为0.013、0.009和0.009。再灌注时间-细胞存活率曲线图提示再灌注2 h 曲线斜率最大，存活率下降最显著。结论：确立心肌细胞缺血再灌注模型缺血时间5 h，再灌注时间2 h。
목적：건립심기세포결혈재관주모형，유조우재세포층면대결혈재관주손상적보호궤제진행탐토，방치림상적무복류현상。방법：분리병순화유서심기세포，배양심기세포지완전동보화。이고순담기화순양분별모의결혈액화재관주액。예설계렬결혈시간화재관주시간，측정세포활력지표제작시간-세포존활솔곡선도，심구최가결혈재관주시간창。결과：심기세포배양제5천박동정동보화，빈솔약90차/ min。여대조조상비，결혈5 h 손상조여기타조적차이유통계학의의(P<0.05)。여대조조상비，재관주2、4화6 h 심기세포존활솔적하강도유통계학차이，P 치분별위0.013、0.009화0.009。재관주시간-세포존활솔곡선도제시재관주2 h 곡선사솔최대，존활솔하강최현저。결론：학립심기세포결혈재관주모형결혈시간5 h，재관주시간2 h。
Objective:The aim of the study is to establish ischemia-reperfusion injury model with myocytes. Methods:Myocytes from neonatal rat are isolated and cultured to beat synchronously for the model of ischemia-reperfusion(I/ R)injury.Nitrogen and oxygen are used to simulate ischemia and reperfusion condition respectively. Then we set series ischemia and reperfusion time windows and drew the time-viability curve to find the best I/ R injury window.Viability of myocytes was measured by CCK,determined as [O.D(Exp) / O.D.(ctrl)]×100%.All continuous variables were expressed as mean±SD.The mean values were compared among groups using one-way ANOVA followed by LSD post hoc analysis. Differences at p < 0. 05 were considered statistically significant. Calculations and statistical tests were performed using SPSS11.0.Results:Myocytes beat synchronously at the fifth day at 90bpm.The group with ischemia for 5 hours reached statistically significance to all other groups(P<0.05). On the ground of ischemia for 5 hours,viability of myocytes with reperfusion at 2,4 and 6 hours all reached statistically significance,P value was 0.013,0.009and 0.009 respectively. The time-viability curve showed the slope of reperfusion for 2 hours was the most prominent,which meant the viability of myocytes fell dramatically at this point.Conclusions:It was concluded that the best I/ R injury model with myocytes should be receive ischemia for 5 hours and reperfusion for 2 hours.