CAJ | 학술논문

作为一种重要的模式识别因子，凝集素在多个领域中均有着广泛的应用价值，因此，开发和利用新型凝集素将具有重要意义．本研究以生活在深海热液区极端环境条件下的管状蠕虫Ridgeia piscesae为材料，首次从管状蠕虫中克隆获得Ricin B-lectin基因 rgal．序列分析表明，RGAL与已知序列的相似性较低，其含有2个Ricin B-lectin型结构域，并且该结构域具有该家族所特有的β-三叶草形三维结构．多方面的分析结果显示RGAL可能是一个新颖的凝集素蛋白．对RGAL进行了原核重组表达，并对其复性条件进行了摸索优化，成功获得了其可溶性表达产物，为后续RGAL的生物活性分析奠定了坚实的基础．
작위일충중요적모식식별인자，응집소재다개영역중균유착엄범적응용개치，인차，개발화이용신형응집소장구유중요의의．본연구이생활재심해열액구겁단배경조건하적관상연충Ridgeia piscesae위재료，수차종관상연충중극륭획득Ricin B-lectin기인 rgal．서렬분석표명，RGAL여이지서렬적상사성교저，기함유2개Ricin B-lectin형결구역，병차해결구역구유해가족소특유적β-삼협초형삼유결구．다방면적분석결과현시RGAL가능시일개신영적응집소단백．대RGAL진행료원핵중조표체，병대기복성조건진행료모색우화，성공획득료기가용성표체산물，위후속RGAL적생물활성분석전정료견실적기출．
Lectin,as an important family of pattern recognition receptors,has been applied widely in various fields. Therefore,development and utilization of new lectins are of great significance.In this study,a Ricin B-lectin gene rgal was first cloned from the tubeworm Ridgeia piscesae,which lives around the hydrothermal vent.Sequence anal-ysis revealed that RGAL had low homology with the known sequence.However,it contained two homologousβ-tre-foil fold typical of the Ricin B-lectin domains.These results suggested that RGAL might be a novel lectin.Further-more,the RGAL was cloned and recombinant expressed and the soluble proteins were obtained.The study will es-tablish a basis for further study on properties and functions of the RGAL.