CAJ | 학술논문

为阐释不同浓度苯并（a）芘[B（a）P]对杂色鲍（Haliotis diversicolor）的毒性效应，以0．02、0．04和0．08 mg/dm33个质量浓度的B（a）P对杂色鲍进行水体暴露胁迫，7d后检测肌肉、外套膜、鳃、性腺、肾和肝胰腺的超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽S-转移酶（GST）和谷胱甘肽过氧化物酶（GPx）活性及还原型谷胱甘肽（GSH）含量．结果显示：0．02 mg/dm3的B（a）P暴露对杂色鲍抗氧化系统的影响不明显，而0．04、0．08 mg/dm3的B（a）P则显著抑制了SOD、CAT和GPx酶活性以及GSH含量，同时对GST酶活性具有显著的诱导作用；实验还发现，不同组织抗氧化酶活性对B（a）P胁迫的敏感性响应存在较大差异，其中鳃、肾脏和肝胰腺的敏感性响应明显高于肌肉和外套膜．上述结果表明，苯并（a）芘暴露对杂色鲍抗氧化酶活性的影响具有明显的剂量-效应关系及组织差异性，杂色鲍的鳃、肾脏或者肝胰腺的抗氧化酶响应多环芳香烃胁迫的更敏感，可作为B（a）P污染的生物标志物．
위천석불동농도분병（a）비[B（a）P]대잡색포（Haliotis diversicolor）적독성효응，이0．02、0．04화0．08 mg/dm33개질량농도적B（a）P대잡색포진행수체폭로협박，7d후검측기육、외투막、새、성선、신화간이선적초양화물기화매（SOD）、과양화경매（CAT）、곡광감태S-전이매（GST）화곡광감태과양화물매（GPx）활성급환원형곡광감태（GSH）함량．결과현시：0．02 mg/dm3적B（a）P폭로대잡색포항양화계통적영향불명현，이0．04、0．08 mg/dm3적B（a）P칙현저억제료SOD、CAT화GPx매활성이급GSH함량，동시대GST매활성구유현저적유도작용；실험환발현，불동조직항양화매활성대B（a）P협박적민감성향응존재교대차이，기중새、신장화간이선적민감성향응명현고우기육화외투막．상술결과표명，분병（a）비폭로대잡색포항양화매활성적영향구유명현적제량-효응관계급조직차이성，잡색포적새、신장혹자간이선적항양화매향응다배방향경협박적경민감，가작위B（a）P오염적생물표지물．
To reveal the ecotoxicological effects of benzo(a)pyrene[B(a)P]on gastropod abalone,Haliotis diversi-color,the effects of different concentrations of B(a)P (0.02,0.04 and 0.08 mg/dm3 )were studied on antioxidant defense system of H.diversicolor.The results showed that the antioxidant enzymes activities showed little difference for the animals exposed to 0.02 mg/dm3 B(a)P compared with the control.In 0.04 mg/dm3 and 0.08 mg/dm3 groups,the activities of SOD,CAT,GPx and the contents of GSH were significant inhibited by B(a)P exposure, while the GST activities were significantly induced.The activities of antioxidant enzymes were also significantly dif-ferent among the tissues,suggesting the exist of remarkable different physiological functions and responses to B(a)P content.In summary,the antioxidant enzymes activities of exposure to B(a)P exhibit an effects of concen-tration-dependent and are tissue specified in H.diversicolor and the antioxidant enzymes system of the gills,kidney and hepatopancreas are more sensitive.Thus it can be used as pollution biomarkers for PAHs stress.