中国临床实用医学
中國臨床實用醫學
중국림상실용의학
CHINA CLINICAL PRACTICAL MEDICINE
2014年
5期
11-14
,共4页
刘兆喆%于明军%郭放%韩涛%韩雅玲%马东初%谢晓冬
劉兆喆%于明軍%郭放%韓濤%韓雅玲%馬東初%謝曉鼕
류조철%우명군%곽방%한도%한아령%마동초%사효동
乳腺癌%斯钙素-1%转移
乳腺癌%斯鈣素-1%轉移
유선암%사개소-1%전이
Breast cancer%Stanniocalcin-1%Metastasis
目的：从mRNA和蛋白水平检测斯钙素-1（STC-1）在乳腺癌细胞系及乳腺癌患者外周血中的表达，并探讨其临床意义。方法2012年7月至2013年1月期间，应用RT-PCR检测3个乳腺癌细胞系、沈阳军区总医院收治的56例乳腺癌患者及10例健康女性外周血STC-1 mRNA的表达；应用酶联免疫吸附实验法检测STC-1蛋白的表达。结果在mRNA和蛋白水平，乳腺癌细胞系及患者外周血中STC-1表达均明显高于健康女性；且在不同TNM分期表达有显著差异（P<0.05），Ⅳ期显著高于Ⅰ、Ⅱ、Ⅲ期（P<0.05）。STC-1 mRNA在小叶癌中的表达较导管癌及混合癌高（P=0.03）。STC-1 mRNA 和蛋白在CEA>5 ng/ml时表达较CEA≤5 ng/ml时明显增高（P<0.05）。结论 STC-1有望成为乳腺癌复发转移的标志物，其表达水平对于乳腺癌预后预测具有重要价值。
目的：從mRNA和蛋白水平檢測斯鈣素-1（STC-1）在乳腺癌細胞繫及乳腺癌患者外週血中的錶達，併探討其臨床意義。方法2012年7月至2013年1月期間，應用RT-PCR檢測3箇乳腺癌細胞繫、瀋暘軍區總醫院收治的56例乳腺癌患者及10例健康女性外週血STC-1 mRNA的錶達；應用酶聯免疫吸附實驗法檢測STC-1蛋白的錶達。結果在mRNA和蛋白水平，乳腺癌細胞繫及患者外週血中STC-1錶達均明顯高于健康女性；且在不同TNM分期錶達有顯著差異（P<0.05），Ⅳ期顯著高于Ⅰ、Ⅱ、Ⅲ期（P<0.05）。STC-1 mRNA在小葉癌中的錶達較導管癌及混閤癌高（P=0.03）。STC-1 mRNA 和蛋白在CEA>5 ng/ml時錶達較CEA≤5 ng/ml時明顯增高（P<0.05）。結論 STC-1有望成為乳腺癌複髮轉移的標誌物，其錶達水平對于乳腺癌預後預測具有重要價值。
목적：종mRNA화단백수평검측사개소-1（STC-1）재유선암세포계급유선암환자외주혈중적표체，병탐토기림상의의。방법2012년7월지2013년1월기간，응용RT-PCR검측3개유선암세포계、침양군구총의원수치적56례유선암환자급10례건강녀성외주혈STC-1 mRNA적표체；응용매련면역흡부실험법검측STC-1단백적표체。결과재mRNA화단백수평，유선암세포계급환자외주혈중STC-1표체균명현고우건강녀성；차재불동TNM분기표체유현저차이（P<0.05），Ⅳ기현저고우Ⅰ、Ⅱ、Ⅲ기（P<0.05）。STC-1 mRNA재소협암중적표체교도관암급혼합암고（P=0.03）。STC-1 mRNA 화단백재CEA>5 ng/ml시표체교CEA≤5 ng/ml시명현증고（P<0.05）。결론 STC-1유망성위유선암복발전이적표지물，기표체수평대우유선암예후예측구유중요개치。
Objective To detect the expression of STC-1 mRNA and protein levels in cell lines and patients of breast cancer, and to investigate the clinical significance. Methods The reverse transcription polymerase chain reaction (RT-PCR) was used to detect STC-1mRNA in 3 breast cancer cell lines, the peripheral blood samples of 56 patients with breast cancer and 10 healthy women. The enzyme linked immunosorbent assay (ELISA) was used to detect STC-1 protein levels in supernatant of breast cancer cell lines, and in serum of 56 patients as well as 10 healthy women. Results The expression of STC-1 of breast cancer cells and patients with breast cancer were statistically higher than that of the healthy women in both mRNA and protein levels (P<0.05). The mRNA and protein levels of STC-1 in patients with breast cancer were related to the TNM stage (P<0.05), both were higher in stageⅣthan in stageⅠ,Ⅱ, andⅢ(P<0.05). The expression of STC-1 mRNA in lobular breast cancer was significantly higher than that in ductal breast cancer and breast mixed carcinoma (P=0.03). STC-1 mRNA and protein levels were higher when CEA>5 ng/ml than those when CEA≤5ng/ml(P<0.05). Conclusion STC-1 could be used as markers of recurrence and metastasis of breast cancer, and might be of great significance in judging prognosis.
