CAJ | 학술논문

蒺藜是一种常用的药用植物，含有多种有效成分，其中总皂苷和总黄酮是其主要成分，总黄酮的含量是总皂苷的1.5倍，因此对蒺藜中总黄酮的提取工艺进行了研究。实验中采用正交设计法对蒺藜中总黄酮的最佳提取工艺进行优化。通过单因素实验得到的初步结果首先确定了四因素，分别为：液固比、提取时间、提取次数和乙醇浓度，同时确定每个因素的三个水平。以总黄酮的含量为考察指标，根据L9(34)正交实验法进行9次实验。研究结果表明：液固比25：1、提取时间10h、提取次数4次、乙醇浓度70%为蒺藜中总黄酮的最佳回流提取工艺。经过结果分析得出：四因素中液固比是实验结果最大影响因素，提取时间对结果也有显著影响，提取次数和乙醇浓度对结果的影响较小。后续验证研究表明该最佳回流提取工艺条件稳定，提取效率较好。
질려시일충상용적약용식물，함유다충유효성분，기중총조감화총황동시기주요성분，총황동적함량시총조감적1.5배，인차대질려중총황동적제취공예진행료연구。실험중채용정교설계법대질려중총황동적최가제취공예진행우화。통과단인소실험득도적초보결과수선학정료사인소，분별위：액고비、제취시간、제취차수화을순농도，동시학정매개인소적삼개수평。이총황동적함량위고찰지표，근거L9(34)정교실험법진행9차실험。연구결과표명：액고비25：1、제취시간10h、제취차수4차、을순농도70%위질려중총황동적최가회류제취공예。경과결과분석득출：사인소중액고비시실험결과최대영향인소，제취시간대결과야유현저영향，제취차수화을순농도대결과적영향교소。후속험증연구표명해최가회류제취공예조건은정，제취효솔교호。
Tribulus is a common medicinal plant that contains a variety of effective ingredients including the total saponins and the total flavonoids are the main ones, and the content of the total flavonoids is 1.5 times more than the saponins. So the extracting technology of the flavonoids from the Tribulus is being studied. We optimize the best extracting technology by the orthogonal design. First depending on the preliminary results which get by the single factor experiments, we choose the four factors, such as the liquid-solid ratio, extraction time, extraction time and concentration of the ethanol, and in the same time we determine the three levels of each factors, and regard the content of total flavonoids as the index. Then conducting nine experiments on the basis of the L9(34) orthogonal design. The results of the studies show that the ratio of liquid to solid and extraction time are 25:1 and 10h, respective. And when tribulus is extracted for 4 times by 70% ethanol, then the total flavonoids has the highest yield. After the analysis of the results, we can draw obtain a conclusion: among the four factors designed in the experiment, the liquid-solid ratio has the greatest impact on the experimental results, the extraction time has a significant influence, and the number of extraction and ethanol concentration have little effect. The later studies show that it is the best reflux extraction process and the extraction efficiency is good.