CAJ | 학술논문

目的：探讨中药复方清肠利肝方对刀豆蛋白A （ Con A）所致急性肝损伤小鼠的防护作用。方法：将野生型C57BL/6（B6）雄性小鼠随机分为正常组、染毒组和清肠利肝方干预组；染毒组通过尾静脉注射Con A （35 mg/kg）诱导肝损伤，干预组先给予清肠利肝方（50 ml/kg）灌胃后再进行染毒。正常组仅给予生理盐水。观察比较各组小鼠的生存率、肝功能水平、组织病理学变化以及相关炎症分子的变化情况。结果：给予小鼠Con A能诱导明显的肝损伤，表现为血浆ALT、 AST水平显著升高，大量肝细胞发生凋亡坏死。清肠利肝方干预组小鼠的ALT、 AST水平低于染毒组，差异有显著性意义（P＜0.05），病理结果显示肝损伤亦得到明显减轻；此外，干预组各项炎症分子如细胞外组蛋白、 IL-6， MCP-1， TNF-α等水平显著低于染毒组，差异有统计学意义（P＜0.05）。结论：清肠利肝方对Con A所致的小鼠急性肝损伤有显著防护作用，其机制可能与减少细胞外组蛋白和相关的细胞因子的水平而达到抗炎的效应有关。
목적：탐토중약복방청장리간방대도두단백A （ Con A）소치급성간손상소서적방호작용。방법：장야생형C57BL/6（B6）웅성소서수궤분위정상조、염독조화청장리간방간예조；염독조통과미정맥주사Con A （35 mg/kg）유도간손상，간예조선급여청장리간방（50 ml/kg）관위후재진행염독。정상조부급여생리염수。관찰비교각조소서적생존솔、간공능수평、조직병이학변화이급상관염증분자적변화정황。결과：급여소서Con A능유도명현적간손상，표현위혈장ALT、 AST수평현저승고，대량간세포발생조망배사。청장리간방간예조소서적ALT、 AST수평저우염독조，차이유현저성의의（P＜0.05），병리결과현시간손상역득도명현감경；차외，간예조각항염증분자여세포외조단백、 IL-6， MCP-1， TNF-α등수평현저저우염독조，차이유통계학의의（P＜0.05）。결론：청장리간방대Con A소치적소서급성간손상유현저방호작용，기궤제가능여감소세포외조단백화상관적세포인자적수평이체도항염적효응유관。
Objective: To investigate the protective effects of a traditional Chinese medicine ( TCM) formula-Qingchang Li-gan against acute liver injury caused by concanavalin A (Con A) in mice.Methods: Male C57BL/6 (B6) mice were randomly divided into three groups:control mice, which received saline only; Con A-treated mice, which was administered with Con A (35mg/kg) via tail vein injection;Qingchang Ligan+Con A-treated mice, which received Qingchang Ligan (50ml/kg) prior to Con A injection.Hepatic function, histological indexes, as well as extracellular histones and the relevant inflammatory cytokines were compared among these groups.Results:Administration of Con A to mice caused acute liver injury, characterized by signifi-cantly increased ALT, AST levels and abnormal histological changes.Qingchang Ligan plus Con A-treated mice showed signifi-cantly lower levels of ALT, AST as well as reduced liver injury than in Con A-treated mice.Besides, the concentrations of extra-cellular histones and IL-6, MCP-1, TNF-αwere markedly decreased in Con A-treated mice after intervention of Qingchang Li-gan formula.Conclusion: Qingchang Ligan formula shows a potent protection against Con A-induced liver injury in mice, likely due to its ability to inhibit the release of extracellular histones and the relevant cytokines.