肿瘤药学
腫瘤藥學
종류약학
ANTI-TUMOR PHARMACY
2014年
5期
336-340
,共5页
舒宝莲%郑新平%王晓洋%伍友兴%桂若虎
舒寶蓮%鄭新平%王曉洋%伍友興%桂若虎
서보련%정신평%왕효양%오우흥%계약호
肝癌%转化生长因子β1%上皮间质转化
肝癌%轉化生長因子β1%上皮間質轉化
간암%전화생장인자β1%상피간질전화
Hepatocellular carcinoma%Transforming growth factor-β1 (TGF-β1)%Epithelial-mesenchymal transition
目的:探讨TGF-β1诱导人肝癌细胞HepG2上皮间质转化及对JAK-STAT3表达的影响。方法采用5μg·mL-1 TGF-β1处理人肝癌细胞HepG2细胞,用倒置显微镜观察细胞形态变化,用Real time-PCR检测E-cadherin和Vimentin的mRNA表达变化;Western blot检测E-cadherin、Vimentin、JAK、p-JAK、STAT3及p-STAT3的蛋白表达变化。结果 TGF-β1可激活JAK-STAT3通路,上调Vimentin表达,同时下调E-cadherin的表达,并显著促进其上皮间质转化。结论 TGF-β1可以诱导人肝癌细胞HepG2上皮间质转化,其机制可能与JAK-STAT3信号通路激活有关。
目的:探討TGF-β1誘導人肝癌細胞HepG2上皮間質轉化及對JAK-STAT3錶達的影響。方法採用5μg·mL-1 TGF-β1處理人肝癌細胞HepG2細胞,用倒置顯微鏡觀察細胞形態變化,用Real time-PCR檢測E-cadherin和Vimentin的mRNA錶達變化;Western blot檢測E-cadherin、Vimentin、JAK、p-JAK、STAT3及p-STAT3的蛋白錶達變化。結果 TGF-β1可激活JAK-STAT3通路,上調Vimentin錶達,同時下調E-cadherin的錶達,併顯著促進其上皮間質轉化。結論 TGF-β1可以誘導人肝癌細胞HepG2上皮間質轉化,其機製可能與JAK-STAT3信號通路激活有關。
목적:탐토TGF-β1유도인간암세포HepG2상피간질전화급대JAK-STAT3표체적영향。방법채용5μg·mL-1 TGF-β1처리인간암세포HepG2세포,용도치현미경관찰세포형태변화,용Real time-PCR검측E-cadherin화Vimentin적mRNA표체변화;Western blot검측E-cadherin、Vimentin、JAK、p-JAK、STAT3급p-STAT3적단백표체변화。결과 TGF-β1가격활JAK-STAT3통로,상조Vimentin표체,동시하조E-cadherin적표체,병현저촉진기상피간질전화。결론 TGF-β1가이유도인간암세포HepG2상피간질전화,기궤제가능여JAK-STAT3신호통로격활유관。
Objective To investigate the effect of TGF-β1 on epithelial-mesenchymal transition and JAK-STAT3 ex-pression in HepG2 cells. Methods HepG2 cells were incubated with 5μg·mL-1 TGF-β1. Cell morphologic changes were observed by microscope. The mRNA expression of E-cadherin and Vimentin were examined by real time-PCR and the pro-tein expressions of E-cadherin, Vimentin, JAK, p-JAK, STAT3, p-STAT3 were detected by western blot. Results TGF-β1 activated the JAK-STAT3 signaling and increased the Vimentin expression. Meanwhile, it decreased the E-cadherin ex-pression and significantly induced EMT in HepG2 cells. Conclusion TGF-β1 could induce EMT in HepG2 cells, and this may be involved with the stimulation of JAK-STAT3 signaling.