云南中医学院学报
雲南中醫學院學報
운남중의학원학보
JOURNAL OF YUNNAN COLLEGE OF TRADITIONAL CHINESE MEDICINE
2014年
5期
16-19,23
,共5页
盛云杰%付玲珠%郑婷%秦磊%张永生
盛雲傑%付玲珠%鄭婷%秦磊%張永生
성운걸%부령주%정정%진뢰%장영생
HPLC%鳖甲煎丸%芍药苷%黄芩苷%丹皮酚
HPLC%鱉甲煎汍%芍藥苷%黃芩苷%丹皮酚
HPLC%별갑전환%작약감%황금감%단피분
HPLC%Biejiajian pills%Paeoniflorin%Baicalin%Paeonol
目的:建立同时测定鳖甲煎丸中芍药苷、黄芩苷、丹皮酚3种有效成分含量的HPLC方法。方法采用Agilent Zorbax Eclipse XDB-C18色谱柱(5μm,4.6mm×150mm),流动相为乙腈(A)和0.1%磷酸水溶液(B),梯度洗脱,流速1.0mL/min;检测波长240、274nm,柱温35℃。结果芍药苷、黄芩苷、丹皮酚线性浓度范围分别是:1.41~14.11μg/mL(r2=0.9999),7.03~70.32μg/mL(r2=0.9999),1.23~12.32μg/mL(r2=0.9997)对照品线性关系良好,各成分的平均回收率(n=9)分别为:100.84%、99.07%、99.08%.结论该方法准确、可靠、重现性好,可用于鳖甲煎丸提取物的质量控制。
目的:建立同時測定鱉甲煎汍中芍藥苷、黃芩苷、丹皮酚3種有效成分含量的HPLC方法。方法採用Agilent Zorbax Eclipse XDB-C18色譜柱(5μm,4.6mm×150mm),流動相為乙腈(A)和0.1%燐痠水溶液(B),梯度洗脫,流速1.0mL/min;檢測波長240、274nm,柱溫35℃。結果芍藥苷、黃芩苷、丹皮酚線性濃度範圍分彆是:1.41~14.11μg/mL(r2=0.9999),7.03~70.32μg/mL(r2=0.9999),1.23~12.32μg/mL(r2=0.9997)對照品線性關繫良好,各成分的平均迴收率(n=9)分彆為:100.84%、99.07%、99.08%.結論該方法準確、可靠、重現性好,可用于鱉甲煎汍提取物的質量控製。
목적:건립동시측정별갑전환중작약감、황금감、단피분3충유효성분함량적HPLC방법。방법채용Agilent Zorbax Eclipse XDB-C18색보주(5μm,4.6mm×150mm),류동상위을정(A)화0.1%린산수용액(B),제도세탈,류속1.0mL/min;검측파장240、274nm,주온35℃。결과작약감、황금감、단피분선성농도범위분별시:1.41~14.11μg/mL(r2=0.9999),7.03~70.32μg/mL(r2=0.9999),1.23~12.32μg/mL(r2=0.9997)대조품선성관계량호,각성분적평균회수솔(n=9)분별위:100.84%、99.07%、99.08%.결론해방법준학、가고、중현성호,가용우별갑전환제취물적질량공제。
Objective To establish a HPLC method for the determinations of paeoniflorin, baicalin and paeonol in Biejiajian pills. Methods Agilent Zorbax Eclipse XDB C18 column (5μm, 4.6 mm ×150 mm), mobile phase of acetonitrile (A)and 0. 1%phosphoric acid (B). Gradient elution, flow rate of 1.0 mL/min, the detection wavelength of 240nm and 274nm, the column temperature was 35℃. Results The linearity ranges of paeoniflorin, baicalin and paeono was 1.41~14.11μg/mL(r2=0.9999), 7.03~70.32μg/mL(r2=0.9999), 1.23 ~12.32μg/mL(r2=0.9997)respectively. The average recoveries(n=9)were100.84%, 99.07%, 99.08%. Conclusion The results showed that the method can be used to control quality of Biejiajian pills.