CAJ | 학술논문

目的：比较卵巢癌SK-OV-3细胞在2D和3D培养系统中的生长特性。方法将卵巢癌细胞株SK-OV-3分别采用2D和3D培养系统进行培养，观察细胞生长形态。采用CCK-8法测定细胞生长，Brdu法检测细胞增殖，流式细胞术检测细胞周期分布。结果 SK-OV-3细胞在2D培养系统中呈单层贴壁生长；在3D培养系统中形成多细胞球样体（muti-cellular spheroid，MCS），生长速度较2D培养系统慢。在2D培养系统中SK-OV-3细胞的S/G2-M期细胞比例为（53．7±5．8）％，明显高于在3D培养系统中S/G2-M期细胞比例[（40．9±2．0）％，P＜0．05]。结论不同的培养方式对卵巢癌细胞的生长具有较大的影响。MCS三维培养系统能更好地模拟体内肿瘤细胞的生长状况，是卵巢癌研究的良好平台。
목적：비교란소암SK-OV-3세포재2D화3D배양계통중적생장특성。방법장란소암세포주SK-OV-3분별채용2D화3D배양계통진행배양，관찰세포생장형태。채용CCK-8법측정세포생장，Brdu법검측세포증식，류식세포술검측세포주기분포。결과 SK-OV-3세포재2D배양계통중정단층첩벽생장；재3D배양계통중형성다세포구양체（muti-cellular spheroid，MCS），생장속도교2D배양계통만。재2D배양계통중SK-OV-3세포적S/G2-M기세포비례위（53．7±5．8）％，명현고우재3D배양계통중S/G2-M기세포비례[（40．9±2．0）％，P＜0．05]。결론불동적배양방식대란소암세포적생장구유교대적영향。MCS삼유배양계통능경호지모의체내종류세포적생장상황，시란소암연구적량호평태。
Objective To compare the growth characteristics of ovarian cancer cells in 2D or 3D culture system. Methods Human ovarian cancer cells of SK-OV-3 were maintained under 2D or 3D culture systems. Cells morphology was observed by the inverted microscope and the growth rate was detected by CCK-8 test re-spectively. The cells ’ proliferation and cycle distribution were analyzed by Brdu assay and flow cytometry (FCM)respectively. Results Maintained under 2D culture system,SK-OV-3 cells grew in monolayer;but under 3D culture system,cells formed multi-cellular spheroids (MCS)with a bit slower growth and proliferation rates. The proportion of S/G2-M phase of cells cultured in 2D system was significantly more than those in 3D system [(53. 7 ± 5. 8)% vs. (40. 9 ± 2. 0)%,P<0. 05]. Conclusions The characteristics of ovarian canc-er cells in 2D and 3D culture systems are different. MCS culture system can better mimic the in vivo growth en-vironment of tumors and could be a better model for ovarian cancer research.