CAJ | 학술논문

为探索氮用量对烤烟碳氮代谢的影响，在前期不同氮素营养水平上在成熟期统一调亏，分析烤烟叶片糖代谢、氮代谢、淀粉代谢及萜类代谢相关关键基因的表达水平。结果显示：糖代谢中3个关键基因，胞外蔗糖转化酶(Inv)和蔗糖合成酶(SuS)基因随着烟叶的成熟表达量升高，在不同氮水平处理间表达水平差异无统计学意义。蔗糖磷酸合成酶(SPS )基因表达量在烟叶成熟前期随之前施氮量的降低而降低。淀粉代谢中颗粒结合型淀粉合成酶( GBSSI )基因表达量既不受烟叶成熟时期的影响也不受前期供氮水平的影响。氮代谢中硝酸还原酶（NR）基因在不同成熟时期及不同的供氮处理间表达量均无差异。谷氨酰胺合成酶（GS）基因的表达量在烟叶成熟后期呈降低趋势，且受前期供氮水平的影响较大。萜类代谢途径中3-羟-3甲基戊二酰辅酶A还原酶（HMGR）基因表达量随着烟叶的成熟先升高再降低，在成熟后期表达量随前期供氮水平的降低而降低。表明在前期供氮水平不一致的基础上于成熟期统一调亏，对成熟后期烟叶氮素的同化吸收过程和萜类代谢影响较大。烟叶糖代谢和淀粉代谢受烟叶成熟时期的影响最大。
위탐색담용량대고연탄담대사적영향，재전기불동담소영양수평상재성숙기통일조우，분석고연협편당대사、담대사、정분대사급첩류대사상관관건기인적표체수평。결과현시：당대사중3개관건기인，포외자당전화매(Inv)화자당합성매(SuS)기인수착연협적성숙표체량승고，재불동담수평처리간표체수평차이무통계학의의。자당린산합성매(SPS )기인표체량재연협성숙전기수지전시담량적강저이강저。정분대사중과립결합형정분합성매( GBSSI )기인표체량기불수연협성숙시기적영향야불수전기공담수평적영향。담대사중초산환원매（NR）기인재불동성숙시기급불동적공담처리간표체량균무차이。곡안선알합성매（GS）기인적표체량재연협성숙후기정강저추세，차수전기공담수평적영향교대。첩류대사도경중3-간-3갑기무이선보매A환원매（HMGR）기인표체량수착연협적성숙선승고재강저，재성숙후기표체량수전기공담수평적강저이강저。표명재전기공담수평불일치적기출상우성숙기통일조우，대성숙후기연협담소적동화흡수과정화첩류대사영향교대。연협당대사화정분대사수연협성숙시기적영향최대。
Expression of key genes involved in sugar, nitrogen, starch and terpenoid metabolism pathways were tested by regulated deficit nitrogen nutrition to study effects of different nitrogen applications on carbon and nitrogen metabolism in flue-cured tobacco. Results indicated that in sugar metabolism pathway Inv and SuS genes’ expression increased when tobacco leaf became more mature while not affected by early nitrogen level. Expression of SPS gene decreased with decline of nitrogen supply at early maturing stage. GBSSI gene in starch metabolism pathway was not affected by either maturity or early nitrogen supply. GS gene expressed at lower level in maturing stage and affected by early nitrogen supply while NR gene expression was not affected. HMGR gene expression in terpenoid metabolism pathway decreased when leaf became more mature and decreased with decline of early nitrogen supply. The assimilation of nitrogen and terpenoid metabolism were significantly affected when regulated deficit of nitrogen was applied at early growing stage. Sugar and starch metabolism were affected mostly at leaf maturing stage.