CAJ | 학술논문

为研究打顶前后烤烟叶片中差异表达的microRNAs (miRNAs)，利用Solexa测序技术对打顶前后烟草叶片的小RNA文库进行测定，分别得到4,223,982和4,993,588条unique sRNA序列读数。打顶前后叶片样品中分别有347条与337条序列与库中miRNA匹配，其中注释序列分别为83和71条。在打顶前后叶片样品中表达具有显著差异的miRNA有7条，在打顶后叶片中高表达的miRNA有miR-157和miR-156；在打顶后叶片中低表达的miRNA有miR-395，miR-159c，miR-159a，miR-394和miR-399。实验还鉴定出3条新的miRNA序列，其中Nta-miRNA*-001和Nta-miRNA*-002存在打顶前叶片样品中，Nta-miRNA*-003存在打顶后叶片中。打顶前后烤烟叶片主要miRNA的表达差异反应了打顶对烤烟叶片的生长及抗逆等应激过程的调节作用。
위연구타정전후고연협편중차이표체적microRNAs (miRNAs)，이용Solexa측서기술대타정전후연초협편적소RNA문고진행측정，분별득도4,223,982화4,993,588조unique sRNA서렬독수。타정전후협편양품중분별유347조여337조서렬여고중miRNA필배，기중주석서렬분별위83화71조。재타정전후협편양품중표체구유현저차이적miRNA유7조，재타정후협편중고표체적miRNA유miR-157화miR-156；재타정후협편중저표체적miRNA유miR-395，miR-159c，miR-159a，miR-394화miR-399。실험환감정출3조신적miRNA서렬，기중Nta-miRNA*-001화Nta-miRNA*-002존재타정전협편양품중，Nta-miRNA*-003존재타정후협편중。타정전후고연협편주요miRNA적표체차이반응료타정대고연협편적생장급항역등응격과정적조절작용。
Expression of microRNAs (miRNAs) profiles in two sRNA libraries which come from flue-cured tobacco leaves before and after topping were shown by Solexa sequencing technology. Totally 4,223,982 and 4,993,588 unique sRNA sequence reads were obtained in before and after topping leaves libraries. The matched miRNAs in before and after leave samples were 347 and 337 in which 83 and 71 were annotated sequences, respectively. Seven miRNAs were identified to be differentially expressed ones between leaves before and after topping. Compared with the expression profiles in before topping samples MiR-157 and MiR-156 were expressed at a relative high level while MiR-395,MiR-159c,MiR-159a,MiR-394 and MiR-399 were shown to be lower expressed. Three new miRNA sequence were predicted in which Nta-miRNA*-001 and Nta-miRNA*-002 were exist in the leave sample before topping and Nta-miRNA*-003 was exist in the after topping sample. The differentially expressed miRNAs indicated that topping can regulate the leaf vegetative growth and resistance ability.