CAJ | 학술논문

为合理调控烟叶蛋白质含量、提高烟叶质量，研究不同温湿度对烟叶蛋白质降解和蛋白酶、内肽酶、谷草转氨酶、谷丙转氨酶活性的变化规律。结果表明，密集烘烤过程中，变黄期不同温湿度下烟叶水分、蛋白质、氨基酸及其相关酶活性的变化规律基本一致；水分和蛋白质含量呈下降趋势，氨基酸含量一直增加，蛋白酶和内肽酶活性呈先升高后降低而后再升高的“双峰曲线”变化；谷丙转氨酶和谷草转氨酶活性在烘烤开始时有小幅度增加，并出现一个小的峰值，然后呈下降趋势，从60 h起急剧升高并达到最大值；且内肽酶活性与可溶性蛋白质相关性显著，和游离氨基酸相关性极显著；谷丙转氨酶只和游离氨基酸相关性显著。密集烘烤低温中湿变黄处理烟叶中性蛋白酶、内肽酶、谷草转氨酶和谷丙转氨酶活性高且作用时间较长，有利于烟叶内蛋白质的降解和氨基酸的合理转化。
위합리조공연협단백질함량、제고연협질량，연구불동온습도대연협단백질강해화단백매、내태매、곡초전안매、곡병전안매활성적변화규률。결과표명，밀집홍고과정중，변황기불동온습도하연협수분、단백질、안기산급기상관매활성적변화규률기본일치；수분화단백질함량정하강추세，안기산함량일직증가，단백매화내태매활성정선승고후강저이후재승고적“쌍봉곡선”변화；곡병전안매화곡초전안매활성재홍고개시시유소폭도증가，병출현일개소적봉치，연후정하강추세，종60 h기급극승고병체도최대치；차내태매활성여가용성단백질상관성현저，화유리안기산상관성겁현저；곡병전안매지화유리안기산상관성현저。밀집홍고저온중습변황처리연협중성단백매、내태매、곡초전안매화곡병전안매활성고차작용시간교장，유리우연협내단백질적강해화안기산적합리전화。
Effects of temperature and humility on protein degradation and activity of protease, endopeptidase, glutamic oxaloacetic transaminase(GOT), glutamic pyruvic transaminase(GPT) were studied. Results showed that the variation trend of content of water, protein and amino acid and related enzyme activities was similar under different temperature and humility in yellowing stage. Contents of water and protein showed downward trend. Content of amino acid showed rising trend. Activities of protease and endopeptidase displayed the bimodal curve. Activities of GOT and GPT increased a little at the start of yellowing before showing downward trend, and then increased drastically from 60h. Endopeptidase activity exhibited significant and highly significant relation with soluble protein and free amino acid respectively. GPT activity had significant relation only with free amino acid. Low temperature and average humility could enable higher activities and longer action time of protease, endopeptidase, GOT and GPT in tobacco leaves in yellowing stage, thus favorable for protein degradation and rational conversion of amino acid.