CAJ | 학술논문

以烟草品种K326为材料，分别种植在河南平顶山、贵州遵义和云南江川三个典型生态区，在烟草叶片发育过程中对叶面腺毛形态、密度、腺毛分泌物及基因表达水平进行了比较研究。显微观察发现腺毛类型在3个地区间变化不大，都以长柄多腺头腺毛为主，但腺毛密度以贵州遵义产区的烟叶最高，河南次之，云南最低；GC/MS检测表明，西柏三烯二醇含量以河南产区为最高，且河南和云南产区随叶龄变化趋势相同；RT-PCR分析表明，西柏烷类化合物合成关键酶基因CYC-1和CYP71D16转录表达水平与西柏三烯二醇含量的变化趋势一致。该研究表明生态环境对烟草腺毛发育和物质代谢具有较大影响，叶面分泌物与烟叶风格特色密切相关。
이연초품충K326위재료，분별충식재하남평정산、귀주준의화운남강천삼개전형생태구，재연초협편발육과정중대협면선모형태、밀도、선모분비물급기인표체수평진행료비교연구。현미관찰발현선모류형재3개지구간변화불대，도이장병다선두선모위주，단선모밀도이귀주준의산구적연협최고，하남차지，운남최저；GC/MS검측표명，서백삼희이순함량이하남산구위최고，차하남화운남산구수협령변화추세상동；RT-PCR분석표명，서백완류화합물합성관건매기인CYC-1화CYP71D16전록표체수평여서백삼희이순함량적변화추세일치。해연구표명생태배경대연초선모발육화물질대사구유교대영향，협면분비물여연협풍격특색밀절상관。
Comparative study was conducted to determain trichomes morphology and densitites, exudates on leaf surface and gene expression by planting one tobacco (Nicotiana tabacum L.) variety K326 in three typical ecological regions, namely Pingdingshan of Henan province, Jiangchuan of Yunnan province, and Zunyi of Guizhou province. Results showed that no obvious difference was observed in trichome types, while trichome densities varied greatly between regions. Leaf trichome in Guizhou’s Zunyi proved to have the highest density, followed by that in Henan and Yunnan. Cembratriene-diol(CBT-diol), the main component of trichome exudates, registered the highest in tobacco leaf surface in Henan according to GC/MS.. The expression of CYC-1 and CYP71D16 displayed same tendency with CBT-diol content. It indicated that ecological environment influenced development of leaf trichomes and metabolism, and further affected characteristics of tobacco leaves.