龙岩学院学报
龍巖學院學報
룡암학원학보
JOURNAL OF LONGYAN UNIVERSITY
2014年
5期
52-57
,共6页
戴婉妹%蔡雅醇%汤灿城%林标声%洪燕萍
戴婉妹%蔡雅醇%湯燦城%林標聲%洪燕萍
대완매%채아순%탕찬성%림표성%홍연평
大孔吸附树脂%枇杷叶粗多糖%蛋白质%多酚%纯化
大孔吸附樹脂%枇杷葉粗多糖%蛋白質%多酚%純化
대공흡부수지%비파협조다당%단백질%다분%순화
macroporous resin%loquat leaf polysaccharide%protein%polyphenol%purification
通过静态吸附和解吸的初步试验考察了六种大孔吸附树脂ADS-7、ADS-17、DM-130、D-101、AB-8、X-5对枇杷叶粗多糖的除蛋白和多酚效果，以蛋白和多酚的吸附率及多糖的回收率为考察指标，筛选出最佳的大孔树脂。结果表明：ADS-7大孔树脂从枇杷多糖提取液中吸附较多蛋白和多酚，同时可以采用10％乙醇洗脱回收多糖，95％乙醇洗脱蛋白质和多酚。因此，采用ADS-7大孔吸附树脂可以通过吸附枇杷粗多糖中杂蛋白和多酚去除杂质，有望获得较高的纯化效率和回收率。
通過靜態吸附和解吸的初步試驗攷察瞭六種大孔吸附樹脂ADS-7、ADS-17、DM-130、D-101、AB-8、X-5對枇杷葉粗多糖的除蛋白和多酚效果，以蛋白和多酚的吸附率及多糖的迴收率為攷察指標，篩選齣最佳的大孔樹脂。結果錶明：ADS-7大孔樹脂從枇杷多糖提取液中吸附較多蛋白和多酚，同時可以採用10％乙醇洗脫迴收多糖，95％乙醇洗脫蛋白質和多酚。因此，採用ADS-7大孔吸附樹脂可以通過吸附枇杷粗多糖中雜蛋白和多酚去除雜質，有望穫得較高的純化效率和迴收率。
통과정태흡부화해흡적초보시험고찰료륙충대공흡부수지ADS-7、ADS-17、DM-130、D-101、AB-8、X-5대비파협조다당적제단백화다분효과，이단백화다분적흡부솔급다당적회수솔위고찰지표，사선출최가적대공수지。결과표명：ADS-7대공수지종비파다당제취액중흡부교다단백화다분，동시가이채용10％을순세탈회수다당，95％을순세탈단백질화다분。인차，채용ADS-7대공흡부수지가이통과흡부비파조다당중잡단백화다분거제잡질，유망획득교고적순화효솔화회수솔。
In this paper, the ability of removing protein and polyphenol from the loquat leaf polysaccharide of six kinds of macroporous resin ADS-7, ADS-17, DM-130, D-101, AB-8 and X-5 was compared through the test of static adsorption and desorption. The best macroporous resin was selected based on the adsorption rate of the protein and polyphenol, and the recovery rate of polysaccharide. The results showed that ADS-7 macroporous resin adsorbed more protein and polyphenol, the polysaccharide could be desorbed by 10% ethanol, and protein and polyphenol could be desorbed by 95% ethanol. Therefore, ADS-7 macroporous adsorption resin can be used to purify loquat polysaccharide through adsorbing protein and polyphenol, and may obtain higher purification efficiency and recovery.
