CAJ | 학술논문

以钙离子荧光指示剂Fluo-3-AM作为神经细胞内Ca2＋荧光探针，用微孔板荧光检测仪测量神经细胞内游离Ca2＋与荧光探针Fluo-3-AM结合的荧光强度，并实时检测此荧光强度在细胞外加不同浓度腺苷诱导下的变化情况。实验发现，各种浓度的腺苷都能诱导神经细胞Ca2＋浓度升高，且其影响程度随腺苷浓度的增大而增强。这一结果对探究腺苷通过作用神经系统起止痛作用的机理具有重要意义。
이개리자형광지시제Fluo-3-AM작위신경세포내Ca2＋형광탐침，용미공판형광검측의측량신경세포내유리Ca2＋여형광탐침Fluo-3-AM결합적형광강도，병실시검측차형광강도재세포외가불동농도선감유도하적변화정황。실험발현，각충농도적선감도능유도신경세포Ca2＋농도승고，차기영향정도수선감농도적증대이증강。저일결과대탐구선감통과작용신경계통기지통작용적궤리구유중요의의。
Calcium ion fluorescent tracers of Fluo-3-AM is used as the Ca2 +fluorescent probe in neurons and the fluorescence intensity combining the intracellular free Ca2+ and the fluorescent probe Fluo-3-AM in neurons is determined by micro-well plate fluorescence detection. And then the fluorescence intensity of the neurons in real-time is determined when the neurons are induced by different concentrations of adenosine. It’ s discovered that the concentration of Ca2+ in neurons is going up slowly when the neurons are induced by adenosine. The influencing degree increases with the increase of the concentration of adenosine. This experiment results are quite important for people to explore the analgesic mechanism of adenosine which affects the function of the nervous system.