基层医学论坛
基層醫學論罈
기층의학론단
PUBLIC MEDICAL FORUM MAGAZINE
2014年
31期
4185-4188
,共4页
魏建勋%马文君%李红梅
魏建勛%馬文君%李紅梅
위건훈%마문군%리홍매
卵巢癌%COC1%米托蒽醌%caspase-3%Mcl-1
卵巢癌%COC1%米託蒽醌%caspase-3%Mcl-1
란소암%COC1%미탁은곤%caspase-3%Mcl-1
Ovarian cancer%COC1%Mitoxantrone%Caspase-3%Mcl-1 gene
目的：研究米托蒽醌（Mitoxantrone，MXT）对人卵巢癌COC1细胞体外增殖与凋亡活性，及凋亡相关基因Mcl-1表达的影响。方法应用四甲基偶氮唑蓝（MTT）法检测不同浓度的MXT对COC1细胞体外增殖活性的影响；流式细胞分析方法（FCM）定量检测不同浓度的MXT作用后COC1细胞的凋亡率；Western-blot检测active caspase-3蛋白和Mcl-1蛋白定量；RT-PCR检测Mcl-1 mRNA的表达变化。结果COC1细胞生长抑制率和凋亡率均随MXT浓度的增加递增（P<0.05）；COC1细胞生长抑制率随MXT作用时间的延长而增高（P<0.05）；active caspase-3蛋白水平随着MXT作用浓度增高递增（P<0.05）；Mcl-1 mRNA和蛋白表达水平随着MXT作用浓度增高递减（P<0.05）。结论米托蒽醌能抑制卵巢癌COC1细胞的增殖，诱导其凋亡，此作用可能与MXT抑制COC1细胞中的Mcl-1表达有关。
目的：研究米託蒽醌（Mitoxantrone，MXT）對人卵巢癌COC1細胞體外增殖與凋亡活性，及凋亡相關基因Mcl-1錶達的影響。方法應用四甲基偶氮唑藍（MTT）法檢測不同濃度的MXT對COC1細胞體外增殖活性的影響；流式細胞分析方法（FCM）定量檢測不同濃度的MXT作用後COC1細胞的凋亡率；Western-blot檢測active caspase-3蛋白和Mcl-1蛋白定量；RT-PCR檢測Mcl-1 mRNA的錶達變化。結果COC1細胞生長抑製率和凋亡率均隨MXT濃度的增加遞增（P<0.05）；COC1細胞生長抑製率隨MXT作用時間的延長而增高（P<0.05）；active caspase-3蛋白水平隨著MXT作用濃度增高遞增（P<0.05）；Mcl-1 mRNA和蛋白錶達水平隨著MXT作用濃度增高遞減（P<0.05）。結論米託蒽醌能抑製卵巢癌COC1細胞的增殖，誘導其凋亡，此作用可能與MXT抑製COC1細胞中的Mcl-1錶達有關。
목적：연구미탁은곤（Mitoxantrone，MXT）대인란소암COC1세포체외증식여조망활성，급조망상관기인Mcl-1표체적영향。방법응용사갑기우담서람（MTT）법검측불동농도적MXT대COC1세포체외증식활성적영향；류식세포분석방법（FCM）정량검측불동농도적MXT작용후COC1세포적조망솔；Western-blot검측active caspase-3단백화Mcl-1단백정량；RT-PCR검측Mcl-1 mRNA적표체변화。결과COC1세포생장억제솔화조망솔균수MXT농도적증가체증（P<0.05）；COC1세포생장억제솔수MXT작용시간적연장이증고（P<0.05）；active caspase-3단백수평수착MXT작용농도증고체증（P<0.05）；Mcl-1 mRNA화단백표체수평수착MXT작용농도증고체감（P<0.05）。결론미탁은곤능억제란소암COC1세포적증식，유도기조망，차작용가능여MXT억제COC1세포중적Mcl-1표체유관。
Objective To investigate the effect of MXT on the proliferation and apoptosis of COC1 cell, and the influence of MXT on the expression of apoptosis related gene Mcl-1 and explore its mechanism.Methods The inhibition effect of different doses of MXT on COC1 cells was assayed with MTT test. COC1 cells apoptosis rate induced by different doses of MXT was detected by FCM method. Western-blotting was used to measure the protein level of active caspase-3 and Mcl-1. The mRNA expression of Mcl-1 in COC1 cells after being exposed to different doses of MXT were determined by RT-PCR. Results The COC1 cell growth inhibitory and apoptosis rate were increased with the increase of the concentration of MXT(P<0.05), The COC1 cell growth inhibitory rate also increased with the duration extension of MXT treatment(P<0.05). The protein level of active caspase-3 was upregulated with the increase of the concentration of MXT (P<0.05). The mRNA and protein expression level of Mcl-1 presented a significant negative correlation with the MXT concentration (P<0.05). Conclusion MXT can inhibit the proliferation of ovarian cancer cell COC1 and induce its apoptosis. This effect may be related to the inhibition of MXT on the mRNA expression of COC1 cell Mcl-1.
