CAJ | 학술논문

载顺铂聚乳酸碳纳米管复合材料对胆管癌细胞株的体外杀伤效应
재순박취유산탄납미관복합재료대담관암세포주적체외살상효응
Anti-tumor effects of DDP-PLLA-CNTs on human cholangiocarcinoma cell line in vitro

万方数据

中华医学杂志中華醫學雜誌 중화의학잡지
National Medical Journal of China
2014年 40期 3163-3166 ,共4页

李茂岚%陆巍%张飞%丁琦晨%吴向嵩%谈竹君%吴文广%翁昊%王雪峰%施伟斌%董平%顾钧%刘颖斌

리무람%륙외%장비%정기신%오향숭%담죽군%오문엄%옹호%왕설봉%시위빈%동평%고균%류영빈

胆管肿瘤%顺铂%聚乳酸%碳纳米管膽管腫瘤%順鉑%聚乳痠%碳納米管
담관종류%순박%취유산%탄납미관
Bile duct neoplasms%Cisplatin%Poly-L-lactic acid%Nanotubes,carbon

目的：制备荷载顺铂的聚乳酸碳纳米管复合材料（ DDP-PLLA-CNTs），并探讨其对人胆管癌细胞株（QBC939）的体外杀伤效应。方法以聚乳酸碳纳米管复合材料（PLLA-CNTs）为原料，采用超声乳化法荷载顺铂（ DDP）；通过电子显微镜观察DDP-PLLA-CNTs形态和结构；用紫外可见光光度仪测定载药量、体外释放量；用CCK8法检测不同浓度的DDP-PLLA-CNTs对QBC939的增殖抑制作用，流式细胞术检测DDP-PLLA-CNTs作用前后细胞凋亡的变化。结果体外释放试验表明DDP-PLLA具有良好的控释特性，DDP-PLLA-CNTs在24 h内有突释行为，释放了29．8％，13 d时释放了49．8％，随后至30 d的体外累积释放率呈缓慢上升趋势，在30 d累积释放率达76．8％。 DDP-PLLA-CNTs可明显抑制 QBC939的增殖活性，并增加胆管癌细胞的凋亡（ DDP-PLLA-CNTs 组凋亡率为81．4％±4．55％，而对照组细胞凋亡率分别为15．4％±1．75％，组间两两比较P＜0．01，差异有统计学意义）。结论 DDP-PLLA-CNTs具有药物缓释效应，并对胆管癌细胞株有明显的杀伤和抑制增殖的作用。
목적：제비하재순박적취유산탄납미관복합재료（ DDP-PLLA-CNTs），병탐토기대인담관암세포주（QBC939）적체외살상효응。방법이취유산탄납미관복합재료（PLLA-CNTs）위원료，채용초성유화법하재순박（ DDP）；통과전자현미경관찰DDP-PLLA-CNTs형태화결구；용자외가견광광도의측정재약량、체외석방량；용CCK8법검측불동농도적DDP-PLLA-CNTs대QBC939적증식억제작용，류식세포술검측DDP-PLLA-CNTs작용전후세포조망적변화。결과체외석방시험표명DDP-PLLA구유량호적공석특성，DDP-PLLA-CNTs재24 h내유돌석행위，석방료29．8％，13 d시석방료49．8％，수후지30 d적체외루적석방솔정완만상승추세，재30 d루적석방솔체76．8％。 DDP-PLLA-CNTs가명현억제 QBC939적증식활성，병증가담관암세포적조망（ DDP-PLLA-CNTs 조조망솔위81．4％±4．55％，이대조조세포조망솔분별위15．4％±1．75％，조간량량비교P＜0．01，차이유통계학의의）。결론 DDP-PLLA-CNTs구유약물완석효응，병대담관암세포주유명현적살상화억제증식적작용。
Objective To explore the antitumor effects of DDP-PLLA-CNTs on human cholangiocarcinoma cell line.Methods DDP-PLLA-CNTs were prepared with the method of ultrasound emulsification.The morphology of DDP-PLLA-CNTs was determined by scanning electron microscope ( SEM ) .And its drug loading and drug release curve in vitro was detected by UV-Vis-NIR spectrophotometer.CCK8 was used to test the cytotoxic effects of DDP-PLLA-CNTs at different concentrations on QBC939 cell proliferation.Flow cytometry was employed to measure the changes of apoptotic rate.Results With excellent controlled-release characteristic of in vitro drug release, DDP-PLLA-CNTs inhibited the proliferation and significantly increased the apoptotic rate of QBC939 cell line.Conclusion DDP-PLLA-CNTs have drug sustained-release characteristics and can significantly inhibit the proliferation of QBC939 cell line.