CAJ | 학술논문

为保护苦苣菜野生资源,以苦苣菜下胚轴为材料,进行了愈伤组织诱导培养、分化培养、生根培养和试管苗移栽及定植的研究,结果表明： MS +琼脂4.8 g·L-1+蔗糖42 g·L-1+ KT 0.4 mg·L-1+ NAA 0.2 mg·L-1+2,4-D2.0 mg·L-1是愈伤组织诱导生长的适宜培养基；1/2 MS+琼脂4.5 g·L-1+蔗糖35 g·L-1+ ZT 0.8 mg·L-1+NAA 0.1 mg·L-1是愈伤组织块分化培养的适宜培养基；1/4 MS+IAA 0.6 mg·L-1+琼脂4.3 g·L-1+蔗糖15 g·L-1是生长芽试管苗培养的适宜培养基；温室内试管苗移栽成活率为91.7%；试管苗在山林旁定植成活率为97.1%,定植试管苗的植物学性状与实生苗基本一致.
위보호고거채야생자원,이고거채하배축위재료,진행료유상조직유도배양、분화배양、생근배양화시관묘이재급정식적연구,결과표명： MS +경지4.8 g·L-1+자당42 g·L-1+ KT 0.4 mg·L-1+ NAA 0.2 mg·L-1+2,4-D2.0 mg·L-1시유상조직유도생장적괄의배양기；1/2 MS+경지4.5 g·L-1+자당35 g·L-1+ ZT 0.8 mg·L-1+NAA 0.1 mg·L-1시유상조직괴분화배양적괄의배양기；1/4 MS+IAA 0.6 mg·L-1+경지4.3 g·L-1+자당15 g·L-1시생장아시관묘배양적괄의배양기；온실내시관묘이재성활솔위91.7%；시관묘재산림방정식성활솔위97.1%,정식시관묘적식물학성상여실생묘기본일치.
In order to preserve the wild resources, with the method of tissue culture, the hypocotyls of Sonchus oleraceus L. were used as materials to do the research on the cultivation of callus inducement, differentiation and rooting, transplantation of tube seedlings and stable planting. The results demonstrated that MS+Agar 4.8 g·L-1+Sucrose 42 g·L-1 + KT 0.4 mg·L-1 + NAA 0.2 mg·L-1 + 2,4-D2.0 mg·L-1 was the ideal medium for callus inducement; 1/2 MS + Agar 4.5 g·L-1+Sucrose 35 g·L-1+ ZT 0.8 mg·L-1+ NAA 0.1 mg·L-1 was the optimum medium for callus differentiation cultivation;The ideal medium for tube seedlings cultivation was 1/4 MS+IAA 0.6 mg·L-1+ Agar 4.3 g·L-1+ Sucrose 15 g·L-1. The transplanting survival rate of tube seedlings was 91.7% in greenhouse. The planting survival rate of tube seedlings was 97.1%. The tube seedlings stable planted maintained all botany characteristics of the wild Sonchus oleraceus L.