北京口腔医学
北京口腔醫學
북경구강의학
BEIJING JOURNAL OF STOMATOLOGY
2014年
5期
251-254
,共4页
毛丽莎%王学玖%葛丽华%宿颖%张春梅
毛麗莎%王學玖%葛麗華%宿穎%張春梅
모려사%왕학구%갈려화%숙영%장춘매
Notch1%牙齿发育%细胞分化%干细胞
Notch1%牙齒髮育%細胞分化%榦細胞
Notch1%아치발육%세포분화%간세포
Notch1%Tooth development%Cell differentiation%Stem cell
目的:探讨Notch1在小鼠下颌第一磨牙胚胎发育过程中的组织学分布。方法制作ICR小鼠下颌第一磨牙不同发育阶段的冰冻组织切片,对小鼠下颌第一磨牙自牙胚发育起始期至出生后2天不同发育阶段组织的Notch1分布情况进行免疫组织化学染色。结果 Notch1在小鼠下颌第一磨牙牙胚发育起始期和蕾状期牙板上皮上方或其包绕的口腔上皮中表达,在牙板上皮中没有表达。自帽状期至钟状期,Notch1在牙胚的中间层表达,而在内釉上皮中无表达。至牙釉质和牙本质分泌期,Notch1仍在颈环部位的中间层表达。此外,Notch1还在牙胚发育不同时期的间充质、牙乳头和早期牙髓中表达。结论 Notch1可能在小鼠下颌第一磨牙发育过程中的牙上皮特别是内釉上皮的细胞分化,以及牙囊和牙乳头细胞分化及分化完成后的牙髓干细胞的稳定性方面有重要作用。
目的:探討Notch1在小鼠下頜第一磨牙胚胎髮育過程中的組織學分佈。方法製作ICR小鼠下頜第一磨牙不同髮育階段的冰凍組織切片,對小鼠下頜第一磨牙自牙胚髮育起始期至齣生後2天不同髮育階段組織的Notch1分佈情況進行免疫組織化學染色。結果 Notch1在小鼠下頜第一磨牙牙胚髮育起始期和蕾狀期牙闆上皮上方或其包繞的口腔上皮中錶達,在牙闆上皮中沒有錶達。自帽狀期至鐘狀期,Notch1在牙胚的中間層錶達,而在內釉上皮中無錶達。至牙釉質和牙本質分泌期,Notch1仍在頸環部位的中間層錶達。此外,Notch1還在牙胚髮育不同時期的間充質、牙乳頭和早期牙髓中錶達。結論 Notch1可能在小鼠下頜第一磨牙髮育過程中的牙上皮特彆是內釉上皮的細胞分化,以及牙囊和牙乳頭細胞分化及分化完成後的牙髓榦細胞的穩定性方麵有重要作用。
목적:탐토Notch1재소서하합제일마아배태발육과정중적조직학분포。방법제작ICR소서하합제일마아불동발육계단적빙동조직절편,대소서하합제일마아자아배발육기시기지출생후2천불동발육계단조직적Notch1분포정황진행면역조직화학염색。결과 Notch1재소서하합제일마아아배발육기시기화뢰상기아판상피상방혹기포요적구강상피중표체,재아판상피중몰유표체。자모상기지종상기,Notch1재아배적중간층표체,이재내유상피중무표체。지아유질화아본질분비기,Notch1잉재경배부위적중간층표체。차외,Notch1환재아배발육불동시기적간충질、아유두화조기아수중표체。결론 Notch1가능재소서하합제일마아발육과정중적아상피특별시내유상피적세포분화,이급아낭화아유두세포분화급분화완성후적아수간세포적은정성방면유중요작용。
Objective To investigate the expression of Notch1 in developing mouse molar tooth germ. Methods Cryosections of developing ICR mouse first molar tooth germ at different developmental stages were made. The expression of Notch1 in the tooth germ tissues was examined by immunohistochemistry. Results Notch1 was expressed in the oral epithelium above or surrounded by the dental placode at initial dental lamina and tooth bud stages, but not expressed in dental placode. From dental cap to bell stage, Notch1 was expressed in stratum intermedium but not in inner enamel epithelium. At enamel and dentin secreting stage, Notch1 was still expressed in the stratum intermedium of the cervical loop. Moreover, Notch1 was also expressed in the mesenchyme, dental papillae and early dental pulp throughout the developing process. Conclusion Notch1 may play an important role in the dental epithelial cell, especially the inner enamel epithelial cell differentiation during the mouse molar tooth germ development and may also be needed in dental follicle, dental papillae and dental pulp cell development and homeostasis.